RRC ID 17700
Author Mitsuyama K, Niwa M, Masuda J, Kuwaki K, Yamasaki H, Takedatsu H, Kobayashi T, Sata M.
Title Isolation and characterization of a novel short peptide associated with Crohn's disease.
Journal Clin. Exp. Immunol.
Abstract Phage display technology has been utilized to select target molecules against circulating antibodies. The aims of this study were to isolate a peptide that binds with serum from Crohn's disease (CD) patients and to examine its diagnostic and pathogenic significance. A phage display library was constructed using cDNA from Caco-2 cells. Affinity selection using this cDNA library and serum samples from patients with CD was then performed. Phage clones that specifically reacted with the CD sera were then selected using a phage enzyme-linked immunosorbent assay (ELISA). After the DNA sequences of the selected phages were determined and converted to amino acid sequences, the synthesized peptides were examined using an ELISA. The effect of the synthesized peptides on cytokine release from cultured blood mononuclear cells was investigated. An ELISA analysis for TCP-353 demonstrated that while 61·7% of the samples from CD patients were seroreactive, seroreactivity was less common among patients with ulcerative colitis (7·3%), acute colitis (0%) or colon cancer (11·4%) and among normal subjects (2·8%). The induction of interleukin (IL)-1β, IL-6 and tumour necrosis factor (TNF)-α release, but not IL-10 release, in response to TCP-353 peptide was enhanced in CD mononuclear cells only. We isolated a novel peptide that specifically binds to CD sera and stimulates the proinflammatory responses of CD mononuclear cells. TCP-353 may have diagnostic, pathogenic and therapeutic significance with regard to the treatment of CD.
Volume 166(1)
Pages 72-9
Published 2011-10
DOI 10.1111/j.1365-2249.2011.04444.x
PMID 21797848
PMC PMC3193921
MeSH Adult Antibodies / blood Antibodies / immunology Caco-2 Cells Case-Control Studies Colitis, Ulcerative / blood Colitis, Ulcerative / diagnosis Colitis, Ulcerative / immunology Colitis, Ulcerative / pathology Colonic Neoplasms / blood Colonic Neoplasms / diagnosis Colonic Neoplasms / immunology Colonic Neoplasms / pathology Crohn Disease / blood* Crohn Disease / diagnosis Crohn Disease / immunology Crohn Disease / pathology DNA, Complementary / analysis Enzyme-Linked Immunosorbent Assay Epitopes Female Humans Interleukin-1beta / biosynthesis* Interleukin-1beta / metabolism Interleukin-6 / biosynthesis* Interleukin-6 / metabolism Leukocytes, Mononuclear / immunology* Leukocytes, Mononuclear / pathology Male Peptide Library Peptides* / chemistry Peptides* / immunology Peptides* / isolation & purification Peptides* / pharmacology ROC Curve Sequence Analysis, DNA Serum / chemistry* Tumor Necrosis Factor-alpha / biosynthesis* Tumor Necrosis Factor-alpha / metabolism
IF 3.542
Times Cited 4
Human and Animal Cells CACO-2 (RCB0988)