Reference - Detail
| RRC ID | 1784 |
|---|---|
| Author | Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S. |
| Title | IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis. |
| Journal | Curr Biol |
| Abstract |
Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKK epsilon is a member of the IkappaB kinase family, and its developmental role has not been clear. Drosophila IKK epsilon was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKK epsilon mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKK epsilon destabilized F actin-based structures. Expression of a dominant-negative form of IKK epsilon increased the amount of DIAP1. The results suggest that at the physiological level, IKK epsilon acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1. |
| Volume | 16(15) |
| Pages | 1531-7 |
| Published | 2006-8-8 |
| DOI | 10.1016/j.cub.2006.06.032 |
| PII | S0960-9822(06)01750-7 |
| PMID | 16887350 |
| MeSH | Actins / metabolism* Animals Apoptosis / physiology* Blotting, Western Cell Differentiation / physiology* Drosophila / physiology* Drosophila Proteins / metabolism* Genetic Testing Histocytochemistry I-kappa B Kinase / metabolism* Inhibitor of Apoptosis Proteins / metabolism* Microscopy, Fluorescence Morphogenesis / physiology* Trachea / cytology Trachea / growth & development |
| IF | 9.601 |
| Times Cited | 73 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| DNA material | pUAST-IKKepsilon (RDB08029) pUAST-IKKepsilonK41A (RDB08030) |
| Drosophila | 2615R-1 2615R-2 12284R-2 8091R-1 8091R-2 DGRC#109662 |