| RRC ID |
1784
|
| Author |
Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.
|
| Title |
IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.
|
| Journal |
Curr Biol
|
| Abstract |
Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKK epsilon is a member of the IkappaB kinase family, and its developmental role has not been clear. Drosophila IKK epsilon was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKK epsilon mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKK epsilon destabilized F actin-based structures. Expression of a dominant-negative form of IKK epsilon increased the amount of DIAP1. The results suggest that at the physiological level, IKK epsilon acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1.
|
| Volume |
16(15)
|
| Pages |
1531-7
|
| Published |
2006-8-8
|
| DOI |
10.1016/j.cub.2006.06.032
|
| PII |
S0960-9822(06)01750-7
|
| PMID |
16887350
|
| MeSH |
Actins / metabolism*
Animals
Apoptosis / physiology*
Blotting, Western
Cell Differentiation / physiology*
Drosophila / physiology*
Drosophila Proteins / metabolism*
Genetic Testing
Histocytochemistry
I-kappa B Kinase / metabolism*
Inhibitor of Apoptosis Proteins / metabolism*
Microscopy, Fluorescence
Morphogenesis / physiology*
Trachea / cytology
Trachea / growth & development
|
| IF |
9.601
|
| Times Cited |
73
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
pUAST-IKKepsilon (RDB08029)
pUAST-IKKepsilonK41A (RDB08030) |
| Drosophila |
2615R-1
2615R-2
12284R-2
8091R-1
8091R-2
DGRC#109662 |
