Reference - Detail
| RRC ID | 1796 |
|---|---|
| Author | Okamura T, Shimizu H, Nagao T, Ueda R, Ishii S. |
| Title | ATF-2 regulates fat metabolism in Drosophila. |
| Journal | Mol Biol Cell |
| Abstract |
ATF-2 is a member of the ATF/CREB family of transcription factors that is activated by stress-activated protein kinases such as p38. To analyze the physiological role of Drosophila ATF-2 (dATF-2), we generated dATF-2 knockdown flies using RNA interference. Reduced dATF-2 in the fat body, the fly equivalent of the mammalian liver and adipose tissue, decreased survival under starvation conditions. This was due to smaller triglyceride reserves of dATF-2 knockdown flies than control flies. Among multiple genes that control triglyceride levels, expression of the Drosophila PEPCK (dPEPCK) gene was strikingly reduced in dATF-2 knockdown flies. PEPCK is a key enzyme for both gluconeogenesis and glyceroneogenesis, which is a pathway required for triglyceride synthesis via glycerol-3-phosphate. Although the blood sugar level in dATF-2 knockdown flies was almost same as that in control flies, the activity of glyceroneogenesis was reduced in the fat bodies of dATF-2 knockdown flies. Thus, reduced glyceroneogenesis may at least partly contribute to decreased triglyceride stores in the dATF-2 knockdown flies. Furthermore we showed that dATF-2 positively regulated dPEPCK gene transcription via several CRE half-sites in the PEPCK promoter. Thus, dATF-2 is critical for regulation of fat metabolism. |
| Volume | 18(4) |
| Pages | 1519-29 |
| Published | 2007-4-1 |
| DOI | 10.1091/mbc.e06-10-0909 |
| PII | E06-10-0909 |
| PMID | 17314398 |
| PMC | PMC1838969 |
| MeSH | Activating Transcription Factor 2 Adipose Tissue / cytology Adipose Tissue / metabolism* Animals Animals, Genetically Modified Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism* Cell Size Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Energy Metabolism Gene Expression Regulation Male Mice Phosphoenolpyruvate Carboxykinase (ATP) / genetics Phosphoenolpyruvate Carboxykinase (ATP) / metabolism RNA Interference Starvation Survival Rate Transcription, Genetic Triglycerides / metabolism |
| Times Cited | 34 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Drosophila | 12850R-2 12850R-3 |