RRC ID 18088
著者 Nakahashi-Oda C, Tahara-Hanaoka S, Shoji M, Okoshi Y, Nakano-Yokomizo T, Ohkohchi N, Yasui T, Kikutani H, Honda S, Shibuya K, Nagata S, Shibuya A.
タイトル Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor.
ジャーナル J Exp Med
Abstract When a cell undergoes apoptosis, phosphatidylserine (PS) is exposed on the outer leaflet of the plasma membrane. PS acts as an "eat-me" signal to direct phagocytes expressing PS receptors to engulf the apoptotic cell. We recently reported that the immunoreceptor CD300a, which is expressed on myeloid cells, is a PS receptor. We show that CD300a does not facilitate macrophage phagocytosis of apoptotic cells. Instead, CD300a delivers an inhibitory signal in mast cells to suppress production of LPS-induced inflammatory cytokines and chemokines. After cecal ligation and puncture (CLP), when a large number of cells undergo apoptosis in the peritoneal cavity, CD300a-deficient peritoneal mast cells produced more chemoattractant and recruited more neutrophils than did wild-type (WT) mast cells. As a result, CD300a-deficient mice showed increased neutrophil recruitment and improved bacterial clearance in the peritoneal cavity, and survived longer than WT mice. Antibody blockade of CD300a-PS interactions improved bacterial clearance and extended survival of WT mice subjected to CLP. These results indicated that CD300a is a nonphagocytic PS receptor that regulates mast cell inflammatory responses to microbial infections.
巻・号 209(8)
ページ 1493-503
公開日 2012-7-30
DOI 10.1084/jem.20120096
PII jem.20120096
PMID 22826299
PMC PMC3409498
MeSH Animals Apoptosis / immunology* Chemokines / immunology Chemokines / metabolism Cytokines / immunology Cytokines / metabolism Humans Inflammation / immunology* Inflammation / metabolism Inflammation / pathology Macrophages / immunology Macrophages / metabolism Mast Cells / immunology* Mast Cells / metabolism Mast Cells / pathology Mice Mice, Inbred BALB C Mice, Inbred C57BL NIH 3T3 Cells Neutrophil Infiltration / immunology Neutrophils / immunology Neutrophils / metabolism Phagocytosis / immunology Phosphatidylserines / immunology Phosphatidylserines / metabolism Receptors, Immunologic / immunology* Receptors, Immunologic / metabolism*
IF 11.743
引用数 51
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY
リソース情報
実験動物マウス RBRC01888