| RRC ID |
18105
|
| 著者 |
Kondo T, Isoda R, Uchimura T, Sugiyama M, Hamao K, Hosoya H.
|
| タイトル |
Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Myosin II is activated by the monophosphorylation of its regulatory light chain (MRLC) at Ser19 (1P-MRLC). Its ATPase activity is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC). As these phosphorylated MRLCs are colocalized with their heavy chains at the contractile ring in dividing cells, we believe that the phosphorylated MRLC acts as a subunit of the activated myosin II during cytokinesis. However, the distinct role(s) of 1P- and 2P-MRLC during cytokinesis has not been elucidated. In this study, a monoclonal antibody (4F12) specific for 2P-MRLC was raised and used to examine the roles of 2P-MRLC in cultured mammalian cells. Our confocal microscopic observations using 4F12 revealed that 2P-MRLC localized to the contractile ring, and, unexpectedly, to the midzone also. Interestingly, 2P-MRLC did not colocalize with 1P-MRLC, myosin II heavy chain, and F-actin at the midzone. These results suggest that 2P-MRLC has a role different from that of 1P-MRLC at the midzone, and is not a subunit of myosin II.
|
| 巻・号 |
417(2)
|
| ページ |
686-91
|
| 公開日 |
2012-1-13
|
| DOI |
10.1016/j.bbrc.2011.11.151
|
| PII |
S0006-291X(11)02192-9
|
| PMID |
22166199
|
| MeSH |
Antibodies, Monoclonal
Cytokinesis*
HeLa Cells
Humans
Myosin Light Chains / genetics
Myosin Light Chains / metabolism*
Phosphorylation
|
| IF |
2.985
|
| 引用数 |
10
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3-3(RCB0150)
HeLa(RCB0007) |
