論文 - 詳細
| RRC ID | 18106 |
|---|---|
| 著者 | Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S. |
| タイトル | The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma. |
| ジャーナル | J Surg Oncol |
| Abstract |
BACKGROUND:CD40 and CD154 are associated with lymphocyte signaling pathways and they are also expressed in some malignant neoplasms, but the significance in pancreatic cancer is unknown. METHODS:Eighty pancreatic cancer specimens were stained immunohistochemically, and the results were correlated with the patients' clinicopathologic features. Subsequently, in vitro analysis of CD40-CD154 signaling was performed. RESULT:Immunohistochemical analysis of tumor cells showed that 29 patients (36.3%) were positive for CD40, and 17 patients (21.3%) had very high CD154 expression. The survival of patients who had very high CD154 expression was significantly better than that of others (P = 0.0198). Univariate and multivariate analysis revealed that very high CD154 expression in cancer cells was not an independent, favorable prognostic factor (risk ratio, 0.493; P = 0.0224). On in vitro proliferation assay, the growth of PK-45P and KP-4 cells was blocked by CD40 and CD154 blocking antibodies. Moreover, on in vitro cytokine assay, Th-2 cytokines from PK-45P and SUIT-2 were blocked by CD40 or CD154 blocking antibody. CONCLUSION:These results suggest that the CD40-CD154 interaction would correlate with cell proliferation and secretion of cytokines in PDAC cells, and CD154 overexpression could be a favorable prognostic factor in PDAC patients. |
| 巻・号 | 103(3) |
| ページ | 230-8 |
| 公開日 | 2011-3-1 |
| DOI | 10.1002/jso.21812 |
| PMID | 21337550 |
| MeSH | Adenocarcinoma / immunology* Adult Aged Aged, 80 and over CD40 Antigens / immunology CD40 Ligand / immunology Carcinoma, Pancreatic Ductal / immunology* Cell Proliferation Female Humans Male Middle Aged Pancreatic Neoplasms / immunology* Signal Transduction Tumor Escape* |
| IF | 2.771 |
| 引用数 | 9 |
| WOS 分野 | SURGERY ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | KP4(RCB1005) MIA Paca2(RCB2094) PANC-1(RCB2095) 1C3D3(RCB0796) HeLa(RCB0007) |