Reference - Detail
| RRC ID | 18109 |
|---|---|
| Author | Hoshi O, Ushiki T. |
| Title | Atomic force microscopy imaging of human metaphase chromosomes in liquid. |
| Journal | Methods Mol Biol |
| Abstract |
Methods for atomic force microscopy (AFM) imaging of human metaphase chromosomes were -introduced in the present study. Chromosomes from the lymphocytes were fixed and prepared onto glass slides as the chromosome spread, and observed in phosphate-buffered saline by dynamic mode AFM. On the contrary, chromosomes from the human cell line BALL-1 were isolated using the hexylene glycol method, absorbed onto a silane-coated glass slide, and observed in a hexylene glycol buffer solution by dynamic mode AFM. AFM provides three-dimensional topographic images of both fixed and unfixed human chromosomes with height information. The ultrastructural image of a pair of chromatids was also obtained by AFM in a liquid condition. The combined use of the AFM and light microscopy of cytochemically and/or immunocytochemically stained chromosomes is also expected to be useful for studies on the localization of chemical components in relation to the higher-order structure of the chromosomes. |
| Volume | 736 |
| Pages | 109-15 |
| Published | 2011-1-1 |
| DOI | 10.1007/978-1-61779-105-5_8 |
| PMID | 21660724 |
| MeSH | Cell Line Chromosomes, Human / chemistry Chromosomes, Human / ultrastructure* Humans Image Processing, Computer-Assisted Metaphase* Microscopy, Atomic Force* |
| Times Cited | 3 |
| WOS Category | MICROSCOPY BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | BALL-1(RCB0256) |