| RRC ID |
18109
|
| Author |
Hoshi O, Ushiki T.
|
| Title |
Atomic force microscopy imaging of human metaphase chromosomes in liquid.
|
| Journal |
Methods Mol Biol
|
| Abstract |
Methods for atomic force microscopy (AFM) imaging of human metaphase chromosomes were -introduced in the present study. Chromosomes from the lymphocytes were fixed and prepared onto glass slides as the chromosome spread, and observed in phosphate-buffered saline by dynamic mode AFM. On the contrary, chromosomes from the human cell line BALL-1 were isolated using the hexylene glycol method, absorbed onto a silane-coated glass slide, and observed in a hexylene glycol buffer solution by dynamic mode AFM. AFM provides three-dimensional topographic images of both fixed and unfixed human chromosomes with height information. The ultrastructural image of a pair of chromatids was also obtained by AFM in a liquid condition. The combined use of the AFM and light microscopy of cytochemically and/or immunocytochemically stained chromosomes is also expected to be useful for studies on the localization of chemical components in relation to the higher-order structure of the chromosomes.
|
| Volume |
736
|
| Pages |
109-15
|
| Published |
2011-1-1
|
| DOI |
10.1007/978-1-61779-105-5_8
|
| PMID |
21660724
|
| MeSH |
Cell Line
Chromosomes, Human / chemistry
Chromosomes, Human / ultrastructure*
Humans
Image Processing, Computer-Assisted
Metaphase*
Microscopy, Atomic Force*
|
| Times Cited |
3
|
|
WOS Category
|
MICROSCOPY
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
BALL-1(RCB0256) |
