RRC ID 18110
著者 Yasuda A, Natsume M, Osakabe N, Kawahata K, Koga J.
タイトル Cacao polyphenols influence the regulation of apolipoprotein in HepG2 and Caco2 cells.
ジャーナル J Agric Food Chem
Abstract Cocoa powder is rich in polyphenols, such as catechins and procyanidins, and has been shown to inhibit low-density lipoprotein (LDL) oxidation and atherogenesis in a variety of models. Human studies have also shown daily intake of cocoa increases plasma high-density lipoprotein (HDL) and decreases LDL levels. However, the mechanisms responsible for these effects of cocoa on cholesterol metabolism have yet to be fully elucidated. The present study investigated the effects of cacao polyphenols on the production of apolipoproteins A1 and B in human hepatoma HepG2 and intestinal Caco2 cell lines. The cultured HepG2 cells or Caco2 cells were incubated for 24 h in the presence of cacao polyphenols such as (-)-epicatechin, (+)-catechin, procyanidin B2, procyanidin C1, and cinnamtannin A2. The concentration of apolipoproteins in the cell culture media was quantified using an enzyme-linked immunoassay, and the mRNA expression was quantified by RT-PCR. Cacao polyphenols increased apolipoprotein A1 protein levels and mRNA expression, even though apolipoprotein B protein and the mRNA expression were slightly decreased in both HepG2 cells and Caco2 cells. In addition, cacao polyphenols increased sterol regulatory element binding proteins (SREBPs) and activated LDL receptors in HepG2 cells. These results suggest that cacao polyphenols may increase the production of mature form SREBPs and LDL receptor activity, thereby increasing ApoA1 and decreasing ApoB levels. These results elucidate a novel mechanism by which HDL cholesterol levels become elevated with daily cocoa intake.
巻・号 59(4)
ページ 1470-6
公開日 2011-2-23
DOI 10.1021/jf103820b
PMID 21226458
MeSH Apolipoprotein A-I / biosynthesis* Apolipoprotein A-I / genetics Apolipoproteins B / biosynthesis* Apolipoproteins B / genetics Cacao / chemistry* Caco-2 Cells Enzyme-Linked Immunosorbent Assay Flavonoids / pharmacology* Hep G2 Cells Humans Phenols / pharmacology* Polyphenols RNA, Messenger / analysis Receptors, LDL / metabolism Reverse Transcriptase Polymerase Chain Reaction Sterol Regulatory Element Binding Proteins / analysis
IF 4.192
引用数 30
WOS 分野 AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED
リソース情報
ヒト・動物細胞 CACO-2(RCB0988) Hep G2(RCB1648)