Reference - Detail
| RRC ID | 18131 |
|---|---|
| Author | Kato J, Kato N, Yoshida H, Ono-Nita SK, Shiratori Y, Omata M. |
| Title | Hepatitis C virus NS4A and NS4B proteins suppress translation in vivo. |
| Journal | J Med Virol |
| Abstract |
Many viruses can inhibit protein synthesis in their host cells by targeting translation ("translational shutoff"). There are few reports on the effects of hepatitis C virus (HCV) infection on protein synthesis, because of the lack of a reproducible tissue culture system for HCV. In this study, the influence of seven HCV proteins (core, NS2, NS3, NS4A, NS4B, NS5A, NS5B) on protein synthesis was examined using a reporter assay. In addition, it was determined whether the HCV proteins inhibit protein synthesis via transcription or translation using an RNase protection assay and the effect of HCV proteins on translation from the HCV internal ribosome entry site (IRES) was also examined using a bicistronic reporter. Of the seven HCV proteins, NS4A and NS4B proteins inhibited cellular protein synthesis by targeting the process of translation. They also inhibited translation from the HCV IRES. Moreover, NS4A protein, induced under the control of doxycycline, inhibited the proliferation of HeLa cells. In conclusion, HCV NS4A and NS4B proteins have an effect of translational inhibition. This novel function may be involved in HCV infection and help its survival in host cells. |
| Volume | 66(2) |
| Pages | 187-99 |
| Published | 2002-2-1 |
| DOI | 10.1002/jmv.2129 |
| PII | 10.1002/jmv.2129 |
| PMID | 11782927 |
| MeSH | Animals COS Cells Chlorocebus aethiops Gene Expression Regulation, Viral Genes, Reporter HeLa Cells Hepacivirus / genetics Hepacivirus / metabolism Hepacivirus / pathogenicity* Hepatitis C / virology* Humans Promoter Regions, Genetic Protein Biosynthesis* Proteins / metabolism Ribonucleases Ribosomes / metabolism Tumor Cells, Cultured Viral Nonstructural Proteins / metabolism* |
| IF | 3.657 |
| Times Cited | 104 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2 HeLa(RCB0007) COS-7(RCB0539) |