論文 - 詳細
| RRC ID | 18144 |
|---|---|
| 著者 | Qi H, Takano H, Kato Y, Wu Q, Ogata C, Zhu B, Murata Y, Nakamura Y. |
| タイトル | Hydrogen [corrected] peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
BACKGROUND:Phloxine B (PhB; 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-fluorescein), an artificial xanthene colorant, has been used as a red coloring agent in drugs and cosmetics as well as foods in some countries. However, little effort has been devoted to the study of this colorant as a potentially useful medicinal agent. METHODS:We investigated the daily light-induced photocytotoxicity of PhB in two human leukemia cells, HL-60 and Jurkat, and its underlying mechanisms by in vitro experiments using antioxidants. REUSLTS AND CONCLUSIONS: PhB inhibited cell proliferation more preferentially to HL-60 cells than to Jurkat cells. Co-treatment of catalase completely blocked the photocytotoxicity by PhB in HL-60 cells, whereas the effect of histidine was only partial, suggesting that hydrogen peroxide (H(2)O(2)), rather than singlet oxygen, might be a prerequisite for the PhB-induced HL-60 cell death. Actually, PhB produced a significant amount of H(2)O(2) in the media as well as in the cells in concentration- and light-dependent manners. Furthermore, methionine, a hypochlorous acid (HOCl) scavenger, also significantly attenuated the cytotoxicity in HL-60 cells, but not in Jurkat cells, indicating the involvement of myeloperoxidase (MPO)-dependent hypohalous acid formation during the photocytotoxicity. In vitro experiments revealed that halogenated tyrosine was generated from the reaction of bovine serum albumin with PhB and HL-60 cell lysate. The present findings suggested that PhB induced a differential photodynamic action in the MPO-containing leukemia cells through an H(2)O(2)-dependent mechanism. GENERAL SIGNIFICANCE:Our findings provide new insights into the molecular mechanisms underlying the PhB-induced apoptosis and also evaluated PhB as a promising PDT agent. |
| 巻・号 | 1810(7) |
| ページ | 704-12 |
| 公開日 | 2011-7-1 |
| DOI | 10.1016/j.bbagen.2011.04.010 |
| PII | S0304-4165(11)00092-4 |
| PMID | 21565256 |
| MeSH | Apoptosis / drug effects Blotting, Western Caspase 3 / metabolism Cell Survival / drug effects* Cell Survival / radiation effects DNA Fragmentation / drug effects Dose-Response Relationship, Drug Eosine I Bluish / chemistry Eosine I Bluish / pharmacology* Fluorescent Dyes / chemistry Fluorescent Dyes / pharmacology Food Coloring Agents / chemistry Food Coloring Agents / pharmacology* HL-60 Cells Humans Hydrogen Peroxide / metabolism* Hydrogen Peroxide / pharmacology Hypochlorous Acid / metabolism Hypochlorous Acid / pharmacology Jurkat Cells Light Molecular Structure Prohibitins Xanthenes / chemistry |
| IF | 3.411 |
| 引用数 | 12 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) |