論文 - 詳細
| RRC ID | 18147 |
|---|---|
| 著者 | Sumitomo T, Nakata M, Higashino M, Jin Y, Terao Y, Fujinaga Y, Kawabata S. |
| タイトル | Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier. |
| ジャーナル | J Biol Chem |
| Abstract |
Group A Streptococcus pyogenes (GAS) is a human pathogen that causes local suppurative infections and severe invasive diseases. Systemic dissemination of GAS is initiated by bacterial penetration of the epithelial barrier of the pharynx or damaged skin. To gain insight into the mechanism by which GAS penetrates the epithelial barrier, we sought to identify both bacterial and host factors involved in the process. Screening of a transposon mutant library of a clinical GAS isolate recovered from an invasive episode allowed identification of streptolysin S (SLS) as a novel factor that facilitates the translocation of GAS. Of note, the wild type strain efficiently translocated across the epithelial monolayer, accompanied by a decrease in transepithelial electrical resistance and cleavage of transmembrane junctional proteins, including occludin and E-cadherin. Loss of integrity of intercellular junctions was inhibited after infection with a deletion mutant of the sagA gene encoding SLS, as compared with those infected with the wild type strain. Interestingly, following GAS infection, calpain was recruited to the plasma membrane along with E-cadherin. Moreover, bacterial translocation and destabilization of the junctions were partially inhibited by a pharmacological calpain inhibitor or genetic interference with calpain. Our data indicate a potential function of SLS that facilitates GAS invasion into deeper tissues via degradation of epithelial intercellular junctions in concert with the host cysteine protease calpain. |
| 巻・号 | 286(4) |
| ページ | 2750-61 |
| 公開日 | 2011-1-28 |
| DOI | 10.1074/jbc.M110.171504 |
| PII | S0021-9258(20)54148-3 |
| PMID | 21084306 |
| PMC | PMC3024771 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism* Caco-2 Cells Cadherins / metabolism Calpain / metabolism Humans Intercellular Junctions / metabolism* Intercellular Junctions / microbiology Pharynx / metabolism Pharynx / microbiology Respiratory Mucosa / metabolism* Respiratory Mucosa / microbiology Streptococcal Infections / enzymology* Streptococcal Infections / genetics Streptococcus pyogenes / enzymology* Streptococcus pyogenes / genetics Streptococcus pyogenes / pathogenicity* Streptolysins / genetics Streptolysins / metabolism* |
| IF | 4.238 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |