| RRC ID |
18158
|
| Author |
Sakai S, Moriyama K, Kawakami K.
|
| Title |
Controlling thermo-reversibility of gelatin gels through a peroxidase-catalyzed reaction under mild conditions for mammalian cells.
|
| Journal |
J Biomater Sci Polym Ed
|
| Abstract |
A variety of cross-linking methods is used for obtaining gelatin gels having a tolerance to thermo-reversible gel-sol transition at physiological temperature. In this paper, we investigated the applicability of horseradish peroxidase-catalyzed cross-linking of tyrosine residues originally contained in native gelatin molecules for preparing such gelatin gels. The gelatin gels obtained through exposure to the enzymatic reaction showed a higher resistance to thermo-reversibility at 37°C than gels obtained through a thermally-induced gelation alone. In addition, the resistance property to thermo-reversible gel-sol transition was tunable by controlling enzymatic reaction conditions: higher peroxidase concentration and thermally-induced pre-gelation accomplished by cooling the gelatin solution prior to the enzymatic reaction produced gels with higher resistance to thermo-reversibility. Fibroblast cells enclosed in the gelatin gels obtained through the enzymatic reaction with thermally-induced pre-gelation showed 93% viability. These results demonstrate the feasibility of peroxidase-catalyzed reaction for obtaining gelatin gels having a tolerance to thermo-reversible gel-to-sol transition at physiological temperature toward applications in biomedical and biopharmaceutical fields.
|
| Volume |
22(9)
|
| Pages |
1147-56
|
| Published |
2011-1-1
|
| DOI |
10.1163/092050610X500589
|
| PII |
jbs3157
|
| PMID |
20615328
|
| MeSH |
Animals
Catalysis
Cell Line
Cell Survival
Feasibility Studies
Fibroblasts / physiology
Gelatin* / chemistry
Horseradish Peroxidase / chemistry
Hydrogels* / chemical synthesis
Hydrogels* / chemistry
Materials Testing
Mice
Swine
Temperature*
Water / chemistry
|
| IF |
2.69
|
| Times Cited |
8
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
POLYMER SCIENCE
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
L929(RCB1451) |
