Reference - Detail
| RRC ID | 18176 |
|---|---|
| Author | Wei K, Li Y, Kim KO, Nakagawa Y, Kim BS, Abe K, Chen GQ, Kim IS. |
| Title | Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior. |
| Journal | J Biomed Mater Res A |
| Abstract |
In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering. |
| Volume | 97(3) |
| Pages | 272-80 |
| Published | 2011-6-1 |
| DOI | 10.1002/jbm.a.33054 |
| PMID | 21442728 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Bombyx / chemistry* Cell Adhesion Cell Proliferation Durapatite / chemistry* Durapatite / metabolism Fibroins / chemistry* Fibroins / metabolism Mice Nanofibers / chemistry* Nanofibers / ultrastructure Osteoblasts / cytology* Osteoblasts / metabolism Tissue Engineering / methods Tissue Scaffolds / chemistry* X-Ray Diffraction |
| IF | 3.525 |
| Times Cited | 97 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |