RRC ID 18181
Author Lee JW, Kang KS, Lee SH, Kim JY, Lee BK, Cho DW.
Title Bone regeneration using a microstereolithography-produced customized poly(propylene fumarate)/diethyl fumarate photopolymer 3D scaffold incorporating BMP-2 loaded PLGA microspheres.
Journal Biomaterials
Abstract Bony defects have been three-dimensionally (3D) created in many clinical circumstances; however, many defects cannot be reconstructed because most of the current bony substitutes cannot provide the necessary exact 3D structure. Therefore, to overcome this limitation, a 3D scaffold with embedded growth factor-delivering microspheres was developed by solid free-form fabrication (SFF) technology using computer-aided design/manufacturing (CAD/CAM). In this study, BMP-2-loaded poly(DL-lactic-co-glycolic acid) (PLGA) microspheres were incorporated into a 3D scaffold that was fabricated using a microstereolithography (MSTL) system with a suspension of microspheres and a poly(propylene fumarate) (PPF)/diethyl fumarate (DEF) photopolymer. By measuring release profiles in vitro, we verified that the fabricated microsphere-containing 3D scaffold could gradually release growth factor. The effects of BMP-2 were also assessed in vitro by observing cell differentiation using MC3T3-E1 pre-osteoblasts. Finally, we confirmed that SFF scaffolds created by MSTL were superior to traditional scaffolds produced using a particulate leaching/gas foaming method. In addition, based on in vivo tests, the scaffolds that released BMP-2 promoted bone formation. Based on these results, we concluded that our 3D scaffold might be a useful tool for enhancing reconstruction quality in many complex bony defects that should be reconstructed using a customized 3D scaffold.
Volume 32(3)
Pages 744-52
Published 2011-1
DOI 10.1016/j.biomaterials.2010.09.035
PII S0142-9612(10)01190-7
PMID 20933279
MeSH Animals Bone Regeneration / physiology* Cell Differentiation / physiology Cell Line Cell Survival / physiology Fumarates / chemistry* Lactic Acid / chemistry* Microspheres* Polyglycolic Acid / chemistry* Polypropylenes / chemistry* Rats Rats, Wistar Tissue Engineering / methods*
IF 8.806
Times Cited 92
Human and Animal Cells MC3T3-E1 (RCB1126)