論文 - 詳細
| RRC ID | 18181 |
|---|---|
| 著者 | Lee JW, Kang KS, Lee SH, Kim JY, Lee BK, Cho DW. |
| タイトル | Bone regeneration using a microstereolithography-produced customized poly(propylene fumarate)/diethyl fumarate photopolymer 3D scaffold incorporating BMP-2 loaded PLGA microspheres. |
| ジャーナル | Biomaterials |
| Abstract |
Bony defects have been three-dimensionally (3D) created in many clinical circumstances; however, many defects cannot be reconstructed because most of the current bony substitutes cannot provide the necessary exact 3D structure. Therefore, to overcome this limitation, a 3D scaffold with embedded growth factor-delivering microspheres was developed by solid free-form fabrication (SFF) technology using computer-aided design/manufacturing (CAD/CAM). In this study, BMP-2-loaded poly(DL-lactic-co-glycolic acid) (PLGA) microspheres were incorporated into a 3D scaffold that was fabricated using a microstereolithography (MSTL) system with a suspension of microspheres and a poly(propylene fumarate) (PPF)/diethyl fumarate (DEF) photopolymer. By measuring release profiles in vitro, we verified that the fabricated microsphere-containing 3D scaffold could gradually release growth factor. The effects of BMP-2 were also assessed in vitro by observing cell differentiation using MC3T3-E1 pre-osteoblasts. Finally, we confirmed that SFF scaffolds created by MSTL were superior to traditional scaffolds produced using a particulate leaching/gas foaming method. In addition, based on in vivo tests, the scaffolds that released BMP-2 promoted bone formation. Based on these results, we concluded that our 3D scaffold might be a useful tool for enhancing reconstruction quality in many complex bony defects that should be reconstructed using a customized 3D scaffold. |
| 巻・号 | 32(3) |
| ページ | 744-52 |
| 公開日 | 2011-1-1 |
| DOI | 10.1016/j.biomaterials.2010.09.035 |
| PII | S0142-9612(10)01190-7 |
| PMID | 20933279 |
| MeSH | Animals Bone Regeneration / physiology* Cell Differentiation / physiology Cell Line Cell Survival / physiology Fumarates / chemistry* Lactic Acid / chemistry* Microspheres* Polyglycolic Acid / chemistry* Polylactic Acid-Polyglycolic Acid Copolymer Polypropylenes / chemistry* Rats Rats, Wistar Tissue Engineering / methods* |
| IF | 10.317 |
| 引用数 | 160 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |