| RRC ID |
18181
|
| 著者 |
Lee JW, Kang KS, Lee SH, Kim JY, Lee BK, Cho DW.
|
| タイトル |
Bone regeneration using a microstereolithography-produced customized poly(propylene fumarate)/diethyl fumarate photopolymer 3D scaffold incorporating BMP-2 loaded PLGA microspheres.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Bony defects have been three-dimensionally (3D) created in many clinical circumstances; however, many defects cannot be reconstructed because most of the current bony substitutes cannot provide the necessary exact 3D structure. Therefore, to overcome this limitation, a 3D scaffold with embedded growth factor-delivering microspheres was developed by solid free-form fabrication (SFF) technology using computer-aided design/manufacturing (CAD/CAM). In this study, BMP-2-loaded poly(DL-lactic-co-glycolic acid) (PLGA) microspheres were incorporated into a 3D scaffold that was fabricated using a microstereolithography (MSTL) system with a suspension of microspheres and a poly(propylene fumarate) (PPF)/diethyl fumarate (DEF) photopolymer. By measuring release profiles in vitro, we verified that the fabricated microsphere-containing 3D scaffold could gradually release growth factor. The effects of BMP-2 were also assessed in vitro by observing cell differentiation using MC3T3-E1 pre-osteoblasts. Finally, we confirmed that SFF scaffolds created by MSTL were superior to traditional scaffolds produced using a particulate leaching/gas foaming method. In addition, based on in vivo tests, the scaffolds that released BMP-2 promoted bone formation. Based on these results, we concluded that our 3D scaffold might be a useful tool for enhancing reconstruction quality in many complex bony defects that should be reconstructed using a customized 3D scaffold.
|
| 巻・号 |
32(3)
|
| ページ |
744-52
|
| 公開日 |
2011-1-1
|
| DOI |
10.1016/j.biomaterials.2010.09.035
|
| PII |
S0142-9612(10)01190-7
|
| PMID |
20933279
|
| MeSH |
Animals
Bone Regeneration / physiology*
Cell Differentiation / physiology
Cell Line
Cell Survival / physiology
Fumarates / chemistry*
Lactic Acid / chemistry*
Microspheres*
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Polypropylenes / chemistry*
Rats
Rats, Wistar
Tissue Engineering / methods*
|
| IF |
10.317
|
| 引用数 |
160
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
