Reference - Detail
| RRC ID | 18209 |
|---|---|
| Author | Watanabe K, Tsunoda K, Itoh M, Fukui M, Mori H, Hitomi K. |
| Title | Transglutaminase 2 and Factor XIII catalyze distinct substrates in differentiating osteoblastic cell line: utility of highly reactive substrate peptides. |
| Journal | Amino Acids |
| Abstract |
Differentiated osteoblastic cell line, MC3T3-E1 expresses transglutaminase 2 (TG2) and Factor XIII (FXIII). In previous studies, we identified isozyme-specific and highly reactive glutamine-donor substrate peptides (pepF11KA and pepT26) for each isozyme. Using these peptides, we compared the reaction products with lysine-donor substrates for each isozyme in differentiating MC3T3-E1 cells. By this analysis, distinct substrates for the activated TG2 and FXIII were detected in cultured cellular extract. Possible substrates that incorporated biotin-labeled peptides were further purified using streptavidin-affinity chromatography. Several isozyme-specific substrates were identified by mass spectrometry analysis of the purified fractions. These analyses also indicate the benefit of the substrate peptides for obtaining distinct substrates in a reaction mixture where two isozymes co-exist. |
| Volume | 44(1) |
| Pages | 209-14 |
| Published | 2013-1-1 |
| DOI | 10.1007/s00726-011-1131-8 |
| PMID | 22045386 |
| MeSH | 3T3 Cells Animals Cell Differentiation* Factor XIII / metabolism* GTP-Binding Proteins / metabolism* Mice Osteoblasts / enzymology* Osteoblasts / physiology Peptide Fragments / metabolism Protein Glutamine gamma Glutamyltransferase 2 Protein Interaction Mapping Substrate Specificity Transglutaminases / metabolism* |
| IF | 3.063 |
| Times Cited | 11 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |