| RRC ID |
18210
|
| 著者 |
Yasui K, Tanabe H, Miyoshi N, Suzuki T, Goto S, Taguchi K, Ishigami Y, Paeng N, Fukutomi R, Imai S, Isemura M.
|
| タイトル |
Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum.
|
| ジャーナル |
Biomed Res
|
| Abstract |
Green tea has been shown to have many beneficial health effects. We have previously reported that dietary (-)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. The present findings suggest EGCG to contribute to the beneficial effects of green tea on diabetes, obesity, and cancer by modulating gene expression in the intestine.
|
| 巻・号 |
32(5)
|
| ページ |
313-20
|
| 公開日 |
2011-10-1
|
| DOI |
10.2220/biomedres.32.313
|
| PII |
JST.JSTAGE/biomedres/32.313
|
| PMID |
22033300
|
| MeSH |
Acetylcysteine / pharmacology
Animals
Antioxidants / administration & dosage
Antioxidants / pharmacology*
Catechin / administration & dosage
Catechin / analogs & derivatives*
Catechin / pharmacology
Cell Line, Tumor
Dose-Response Relationship, Drug
Duodenum / drug effects*
Duodenum / metabolism*
Gene Expression Regulation / drug effects*
Gluconeogenesis / drug effects*
Gluconeogenesis / genetics*
Humans
Mice
|
| IF |
1.429
|
| 引用数 |
14
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| リソース情報 |
| ヒト・動物細胞 |
CACO-2(RCB0988) |
