論文 - 詳細
| RRC ID | 18216 |
|---|---|
| 著者 | Mogi M, Kondo A. |
| タイトル | The presence of TRAIL-OPG complex in human osteosarcoma and human salivary gland adenocarcinoma. |
| ジャーナル | J Immunoassay Immunochem |
| Abstract |
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the human tumor necrosis factor-related apoptosis-induced ligand (TRAIL)-osteoprotegerin (OPG) complex was developed by utilizing a monoclonal antibody that recognizes human soluble TRAIL as an immobilized capture component and anti-human OPG immunoglobulin G (IgG) labeled with biotin. We could quantify the TRAIL-OPG complex level (detection limit: 10 pmol/L). Employing this assay system, we demonstrated that the TRAIL-OPG complex was constitutively present in the human osteosarcoma cell lines, HOS, MG-63, and human salivary gland adenocarcinoma cell line, HSG. |
| 巻・号 | 32(1) |
| ページ | 70-8 |
| 公開日 | 2011-1-1 |
| DOI | 10.1080/15321819.2010.538110 |
| PII | 932570306 |
| PMID | 21253971 |
| MeSH | Adenocarcinoma / immunology* Antibodies, Monoclonal / immunology Cell Line, Tumor Enzyme-Linked Immunosorbent Assay / methods* Humans Osteosarcoma / immunology* Osteosarcoma / metabolism RANK Ligand / analysis RANK Ligand / immunology* Salivary Gland Neoplasms / immunology* TNF-Related Apoptosis-Inducing Ligand / analysis TNF-Related Apoptosis-Inducing Ligand / immunology* |
| 引用数 | 2 |
| WOS 分野 | MEDICAL LABORATORY TECHNOLOGY BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) |