Reference - Detail
RRC ID | 18218 |
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Author | Ohtani M, Miyadai T. |
Title | Functional analysis of fish BCL-6 and Blimp-1 in vitro: transcriptional repressors for B-cell terminal differentiation in fugu (Takifugu rubripes). |
Journal | Mol Immunol |
Abstract |
The transcriptional repressors BCL-6 and Blimp-1 are key regulators of B-cell terminal differentiation in mammals. We have previously identified the BCL-6 gene and Blimp-1 gene in fugu (Takifugu rubripes). In the present report, we conducted a functional analysis of fugu BCL-6 and Blimp-1 by using a one-hybrid reporter assay with Gal4 fusion proteins and Gal4DBD luciferase reporter gene. Results from the reporter assays in mammalian cell lines (HeLa, HEK-293, CV-1 and NIH3T3) and the fish cell line EPC show that Gal4-BCL6 and Gal4-Blimp1 strongly repress the transcription of the luciferase gene in all cell lines. Furthermore, deletion analyses show that the N-terminal region of BCL-6 has transcriptional repression activity; the BTB/POZ domain is an especially potent repression domain. In contrast to BCL-6, although the N-acidic domain and PR domain are insufficient for repression, most functional motifs of Blimp-1 are associated with transcriptional repression. These results suggest that BCL-6 and Blimp-1 are functional transcriptional repressors in fugu and that they regulate B-cell terminal differentiation in fugu. |
Volume | 48(6-7) |
Pages | 818-25 |
Published | 2011-3-1 |
DOI | 10.1016/j.molimm.2010.10.018 |
PII | S0161-5890(10)00598-5 |
PMID | 21216469 |
MeSH | Amino Acid Motifs Animals B-Lymphocytes / cytology* B-Lymphocytes / metabolism Cell Differentiation / genetics* Cell Line Fish Proteins / chemistry Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation HeLa Cells Humans Mice NIH 3T3 Cells Protein Structure, Tertiary Proto-Oncogene Proteins c-bcl-6 / chemistry Proto-Oncogene Proteins c-bcl-6 / genetics Proto-Oncogene Proteins c-bcl-6 / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Sequence Deletion Takifugu / genetics* Transcription, Genetic* |
IF | 3.641 |
Times Cited | 9 |
WOS Category | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | 293(RCB1637) |