論文 - 詳細
| RRC ID | 1822 |
|---|---|
| 著者 | Miyauchi K, Ohara T, Suzuki T. |
| タイトル | Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Recent genome-wide transcriptome analysis has identified diverse classes of non-coding RNAs (ncRNAs), some of which have been demonstrated to be functional, regulatory RNAs involved in various biological processes. Maturation of RNA molecules through various post-transcriptional processing events, including splicing, modification, editing and trimming of both ends, is required for correct folding and proper function of RNA molecules. To characterize post-transcriptional modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. However, there have been no general and convenient strategies for isolation of individual RNAs. We describe here the first example of automated parallel isolation of individual ncRNAs using a novel method named 'reciprocal circulating chromatography (RCC)'. RCC employs multiple tip-columns packed with solid-phase DNA probes to isolate multiple RNA species from a common sample of total RNAs. A pilot RCC instrument successfully isolated various ncRNAs from E. coli, yeast and mouse. |
| 巻・号 | 35(4) |
| ページ | e24 |
| 公開日 | 2007-1-1 |
| DOI | 10.1093/nar/gkl1129 |
| PII | gkl1129 |
| PMID | 17251194 |
| PMC | PMC1851638 |
| MeSH | Animals Chromatography, Affinity / instrumentation Chromatography, Affinity / methods* DNA Probes Escherichia coli / genetics Mice RNA, Bacterial / isolation & purification RNA, Fungal / isolation & purification RNA, Transfer / isolation & purification RNA, Untranslated / isolation & purification* Saccharomyces cerevisiae / genetics |
| IF | 11.502 |
| 引用数 | 52 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 情報 | E.coli |