| RRC ID |
18225
|
| 著者 |
Hasegawa S, Koshikawa-Yano M, Saito S, Morokoshi Y, Furukawa T, Aoki I, Saga T.
|
| タイトル |
Molecular imaging of mesothelioma by detection of manganese-superoxide dismutase activity using manganese-enhanced magnetic resonance imaging.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
Malignant mesothelioma (MM) is a fatal malignancy with a rapidly increasing incidence in industrialized countries because of the widespread use of asbestos in the past centuries. Early diagnosis of MM is critical for a better prognosis, but this is often difficult because of the lack of disease-specific diagnostic imaging. Here, we report that manganese-enhanced magnetic resonance imaging (MEMRI) represents a promising approach for a more selective mesothelioma imaging by monitoring a high-level expression of manganese-superoxide dismutase (Mn-SOD), which is observed in many MM. We found that most human MM cells overexpressed Mn-SOD protein compared with human mesothelial cells and that NCI-H226 human MM cells highly expressed Mn-SOD and augmented Mn accumulation when loaded with manganese chloride (MnCl(2)). The cells showed marked T(1)-signal enhancement on in vitro MRI after incubation with MnCl(2) because of the T(1) shortening effect of Mn(2+). H226 subcutaneous tumor was preferentially enhanced compared with a lung adenocarcinoma cell tumor and another human MM cell tumor in MnCl(2)-enhanced T(1)-weighted MR image (T(1)WI), correlating with their respective Mn-SOD expression levels. Moreover, in a more clinically relevant setting, H226 xenografted pleural tumor was markedly enhanced and readily detected by MEMRI using manganese dipyridoxyl diphosphate (MnDPDP), a clinically used contrast agent, as well as MnCl(2). Therefore, we propose that MEMRI can be a potentially powerful method for noninvasive detection of MM, with high spatial resolution and marked signal enhancement, by targeting Mn-SOD.
|
| 巻・号 |
128(9)
|
| ページ |
2138-46
|
| 公開日 |
2011-5-1
|
| DOI |
10.1002/ijc.25547
|
| PMID |
20617513
|
| MeSH |
Animals
Blotting, Western
Cell Line, Tumor
Disease Models, Animal
Humans
Immunohistochemistry
Magnetic Resonance Imaging / methods*
Mesothelioma / diagnosis*
Mesothelioma / enzymology*
Mice
Mice, Nude
Pleural Neoplasms / diagnosis
Pleural Neoplasms / enzymology
Reverse Transcriptase Polymerase Chain Reaction
Superoxide Dismutase / metabolism*
|
| IF |
5.145
|
| 引用数 |
14
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ACC-MESO-1(RCB2292)
ACC-MESO-4(RCB2293)
HMMME(RCB0819) |
