| RRC ID |
18237
|
| Author |
Yamamoto H, Abe Y, Miyazato A, Tanno D, Tanaka M, Miyasaka T, Ishii K, Kawakami K.
|
| Title |
Cryptococcus neoformans suppresses the activation of bone marrow-derived dendritic cells stimulated with its own DNA, but not with DNA from other fungi.
|
| Journal |
FEMS Immunol Med Microbiol
|
| Abstract |
DNA from Cryptococcus neoformans activates bone marrow-derived dendritic cells (BM-DCs) in a TLR9-dependent manner. In this study, we examined the effect of the culture supernatants of C. neoformans on the activation of BM-DCs caused by its own DNA. C. neoformans supernatants suppressed IL-12p40, IL-6 production and CD40 expression by BM-DCs stimulated with its own DNA, but not with CpG-ODN and DNA from Candida albicans, Saccharomyces cerevisiae or Escherichia coli. In a confocal microscopic analysis, C. neoformans DNA was colocalized with LAMP-1, a late endosomal marker, and TLR9. The culture supernatants did not show any apparent suppression of these responses. In a luciferase reporter assay, C. neoformans supernatants inhibited NFκB activation caused by its own DNA. These inhibitory activities were attenuated by treatment with heat or trypsin. These results indicate that C. neoformans secrete certain proteinous molecules that suppress the activation of BM-DCs caused by its own DNA.
|
| Volume |
63(3)
|
| Pages |
363-72
|
| Published |
2011-12-1
|
| DOI |
10.1111/j.1574-695X.2011.00859.x
|
| PMID |
22092563
|
| MeSH |
Animals
Bone Marrow / immunology*
CD40 Antigens / antagonists & inhibitors
CD40 Antigens / metabolism
Candida albicans / immunology
Cryptococcus neoformans / immunology*
Cryptococcus neoformans / pathogenicity*
DNA, Fungal / immunology
DNA, Fungal / metabolism*
Dendritic Cells / immunology*
Dendritic Cells / microbiology*
Escherichia coli / immunology
Immune Tolerance
Interleukin-12 Subunit p40 / antagonists & inhibitors
Interleukin-12 Subunit p40 / metabolism
Interleukin-6 / antagonists & inhibitors
Interleukin-6 / metabolism
Male
Mice
Mice, Inbred C57BL
Saccharomyces cerevisiae / immunology
|
| Times Cited |
6
|
|
WOS Category
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
