RRC ID 18244
Author Yang CL, Chen HW, Wang TC, Wang YJ.
Title A novel fibrin gel derived from hyaluronic acid-grafted fibrinogen.
Journal Biomed Mater
Abstract Fibrinogen is a major plasma protein that forms a three-dimensional fibrin gel upon being activated by thrombin. In this study, we report the synthesis and potential applications of hybrid molecules composed of fibrinogen coupled to the reducing ends of short-chain hyaluronic acids (sHAs) by reductive amination. The grafting of sHAs to fibrinogen was verified by analyzing particle size, zeta potential and gel-electrophoretic mobility of the hybrid molecules. The sHA-fibrinogen hybrid molecules with graft ratios (sHA/fibrinogen) of up to 6.5 retained the ability to form gels in response to thrombin activation. The sHA-fibrin gels were transparent in appearance and exhibited high water content, which were characteristics distinct from those of gels formed by mixtures of sHAs and fibrinogen. The potential applications of the sHA-fibrin gels were evaluated. The sHA-fibrinogen gel with a graft ratio of 3.6 (S3.6F) was examined for its ability to encapsulate and support the differentiation of ATDC5 chondrocyte-like cells. Compared with the fibrinogen-formed gel, cells cultured in the S3.6F gel exhibited increased lacunae formation; moreover, the abundance of cartilaginous extracellular matrix molecules and the expression of chondrocyte marker genes, such as aggrecan, collagen II and Sox9, were also significantly increased. Our data suggest that the three-dimensional gel formed by the sHA-fibrinogen hybrid is a better support than the fibrin gel for chondrogenesis induction.
Volume 6(2)
Pages 025009
Published 2011-4-1
DOI 10.1088/1748-6041/6/2/025009
PII S1748-6041(11)74359-5
PMID 21393811
MeSH Animals Biocompatible Materials / chemistry Blood Proteins / chemistry Cartilage / metabolism Cell Differentiation Cell Line Chondrocytes / cytology Extracellular Matrix / metabolism Fibrin / chemistry* Fibrinogen / chemistry* Gels / chemistry Hyaluronic Acid / chemistry* Mice Microscopy, Electron, Scanning Models, Chemical Particle Size Thrombin / chemistry Water / chemistry
IF 3.44
Times Cited 10
Human and Animal Cells ATDC5 (RCB0565)