論文 - 詳細
| RRC ID | 18250 |
|---|---|
| 著者 | Handa T, Anada T, Honda Y, Yamazaki H, Kobayashi K, Kanda N, Kamakura S, Echigo S, Suzuki O. |
| タイトル | The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects. |
| ジャーナル | Acta Biomater |
| Abstract |
This study was designed to investigate the extent to which an octacalcium phosphate/gelatin (OCP/Gel) composite can repair rat calvarial critical-sized defects (CSD). OCP crystals were grown with various concentrations of gelatin molecules and the OCP/Gel composites were characterized by chemical analysis, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), selected area electron diffraction (SAED) and mercury intrusion porosimetry. The OCP/Gel composite disks received vacuum dehydrothermal treatment, were implanted in Wistar rat calvarial CSD for 4, 8 and 16 weeks, and then subjected to radiologic, histologic, histomorphometric and histochemical assessment. The attachment of mouse bone marrow stromal ST-2 cells on the disks of the OCP/Gel composites was also examined after 1 day of incubation. OCP/Gel composites containing 24 wt.%, 31 wt.% and 40 wt.% of OCP and with approximate pore sizes of 10-500 μm were obtained. Plate-like crystals were observed closely associated with the Gel matrices. TEM, XRD, FTIR and SAED confirmed that the plate-like crystals were identical to those of the OCP phase, but contained a small amount of sphere-like amorphous material adjacent to the OCP crystals. The OCP (40 wt.%)/Gel composite repaired 71% of the CSD in conjunction with material degradation by osteoclastic cells, which reduced the percentage of the remaining implant to less than 3% within 16 weeks. Of the seeded ST-2 cells, 60-70% were able to migrate and attach to the OCP/Gel composites after 1 day of incubation, regardless of the OCP content. These results indicate that an OCP/Gel composite can repair rat calvarial CSD very efficiently and has favorable biodegradation characteristics. Therefore, it is hypothesized that host osteoblastic cells can easily migrate into an OCP/Gel composite. |
| 巻・号 | 8(3) |
| ページ | 1190-200 |
| 公開日 | 2012-3-1 |
| DOI | 10.1016/j.actbio.2011.12.002 |
| PII | S1742-7061(11)00538-1 |
| PMID | 22198138 |
| MeSH | Absorbable Implants* Animals Bone Marrow Cells / metabolism Bone Marrow Cells / pathology Bone Substitutes / chemistry Bone Substitutes / pharmacology* Calcium Phosphates / chemistry Calcium Phosphates / pharmacology* Cell Line Gelatin / chemistry Gelatin / pharmacology* Male Materials Testing* Mice Microscopy, Electron, Transmission Rats Rats, Wistar Skull / injuries* Skull / metabolism Skull / pathology Skull Fractures / metabolism Skull Fractures / pathology Skull Fractures / therapy* Spectroscopy, Fourier Transform Infrared Stromal Cells / metabolism Stromal Cells / pathology |
| IF | 7.242 |
| 引用数 | 33 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |