| RRC ID |
18510
|
| 著者 |
Kihara T, Ichikawa S, Yonezawa T, Lee JW, Akihisa T, Woo JT, Michi Y, Amagasa T, Yamaguchi A.
|
| タイトル |
Acerogenin A, a natural compound isolated from Acer nikoense Maxim, stimulates osteoblast differentiation through bone morphogenetic protein action.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We investigated the effects of acerogenin A, a natural compound isolated from Acer nikoense Maxim, on osteoblast differentiation by using osteoblastic cells. Acerogenin A stimulated the cell proliferation of MC3T3-E1 osteoblastic cells and RD-C6 osteoblastic cells (Runx2-deficient cell line). It also increased alkaline phosphatase activity in MC3T3-E1 and RD-C6 cells and calvarial osteoblastic cells isolated from the calvariae of newborn mice. Acerogenin A also increased the expression of mRNAs related to osteoblast differentiation, including Osteocalcin, Osterix and Runx2 in MC3T3-E1 cells and primary osteoblasts: it also stimulated Osteocalcin and Osterix mRNA expression in RD-C6 cells. The acerogenin A treatment for 3days increased Bmp-2, Bmp-4, and Bmp-7 mRNA expression levels in MC3T3-E1 cells. Adding noggin, a BMP specific-antagonist, inhibited the acerogenin A-induced increase in the Osteocalcin, Osterix and Runx2 mRNA expression levels. These results indicated that acerogenin A stimulates osteoblast differentiation through BMP action, which is mediated by Runx2-dependent and Runx2-independent pathways.
|
| 巻・号 |
406(2)
|
| ページ |
211-7
|
| 公開日 |
2011-3-11
|
| DOI |
10.1016/j.bbrc.2011.02.017
|
| PII |
S0006-291X(11)00200-2
|
| PMID |
21303661
|
| MeSH |
Acer / chemistry*
Animals
Bone Morphogenetic Proteins / biosynthesis*
Bone Regeneration / drug effects*
Carrier Proteins / metabolism
Cell Differentiation / drug effects*
Cell Line
Core Binding Factor Alpha 1 Subunit / biosynthesis
Diarylheptanoids / chemistry
Diarylheptanoids / isolation & purification
Diarylheptanoids / pharmacology*
Mice
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteocalcin / biosynthesis
Phenyl Ethers / chemistry
Phenyl Ethers / isolation & purification
Phenyl Ethers / pharmacology*
RNA, Messenger / biosynthesis
Sp7 Transcription Factor
Transcription Factors / biosynthesis
|
| IF |
2.985
|
| 引用数 |
8
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
