論文 - 詳細
| RRC ID | 18516 |
|---|---|
| 著者 | Kang KS, Lee SJ, Lee HS, Moon W, Cho DW. |
| タイトル | Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts. |
| ジャーナル | Exp Mol Med |
| Abstract |
We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and ultrasound, each of which is known to effectively stimulate bone tissue regeneration. MC3T3-E1 pre-osteoblasts were chosen for bone tissue engineering due to their osteoblast-like characteristics. 3-D scaffolds were fabricated with polycaprolactone and poly-L-lactic acid using the salt leaching method. The cells were stimulated by the bioreactor with cyclic strain and ultrasound. The bioreactor was set at a frequency of 1.0 Hz and 10 % strain for cyclic strain and 1.0 MHz and 30 mW/cm(2) for ultrasound. Three experimental groups (ultrasound, cyclic strain, and combined stimulation) and a control group were examined. Each group was stimulated for 20 min/day. Mechanical stimuli did not affect MC3T3-E1 cell proliferation significantly up to 10 days when measured with the cell counting kit-8. However, gene expression analysis of collagen type-I, osteocalcin, RUNX2, and osterix revealed that the combined mechanical stimulation accelerated the matrix maturation of MC3T3-E1 cells. These results indicate that the combined mechanical stimulation can enhance the differentiation of pre-osteoblasts more efficiently than simple stimuli, in spite of no effect on cell proliferation. |
| 巻・号 | 43(6) |
| ページ | 367-73 |
| 公開日 | 2011-6-30 |
| DOI | 10.3858/emm.2011.43.6.040 |
| PII | emm.2010.43.040 |
| PMID | 21532314 |
| PMC | PMC3128915 |
| MeSH | Animals Bioreactors Bone Regeneration* Cell Differentiation Cell Line Cell Proliferation Lactic Acid / chemistry Mechanical Phenomena* Mechanotransduction, Cellular / physiology Mice Osteoblasts / cytology Osteoblasts / metabolism* Polyesters / chemistry Polymers / chemistry Tissue Engineering / methods Tissue Scaffolds / chemistry Tissue Scaffolds / statistics & numerical data |
| IF | 5.418 |
| 引用数 | 32 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |