論文 - 詳細
| RRC ID | 18519 |
|---|---|
| 著者 | Chatterjee K, Young MF, Simon CG Jr. |
| タイトル | Fabricating gradient hydrogel scaffolds for 3D cell culture. |
| ジャーナル | Comb Chem High Throughput Screen |
| Abstract |
Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels. |
| 巻・号 | 14(4) |
| ページ | 227-36 |
| 公開日 | 2011-5-1 |
| DOI | 10.2174/138620711795222455 |
| PII | BSP/CCHTS/E- Pub/00137 |
| PMID | 21143178 |
| PMC | PMC3381353 |
| MeSH | Animals Biocompatible Materials / chemistry* Biocompatible Materials / metabolism Biomechanical Phenomena Bone Regeneration Cell Culture Techniques / methods* Cell Differentiation Cell Line Cell Survival Elastic Modulus High-Throughput Screening Assays Hydrogels / chemistry* Hydrogels / metabolism Mice Osteoblasts / cytology* Osteoblasts / metabolism Polyethylene Glycols / chemistry* Polyethylene Glycols / metabolism Tissue Engineering / methods* Tissue Scaffolds Trypan Blue / analysis |
| IF | 1.195 |
| 引用数 | 15 |
| WOS 分野 | PHARMACOLOGY & PHARMACY BIOCHEMICAL RESEARCH METHODS CHEMISTRY, APPLIED |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |