論文 - 詳細
| RRC ID | 18543 |
|---|---|
| 著者 | Okada E, Yamanaka M, Ishikawa O. |
| タイトル | New insights into the mechanism of abnormal calcification in nephrogenic systemic fibrosis - gadolinium promotes calcium deposition of mesenchymal stem cells and dermal fibroblasts. |
| ジャーナル | J Dermatol Sci |
| Abstract |
BACKGROUND:Recent studies have suggested that there is a close association between the administration of gadolinium (Gd)-based contrast agents and the development of nephrogenic systemic fibrosis (NSF), an acquired disorder characterized by systemic fibrosis and abnormal calcification in patients with severe renal dysfunction. However, the causative roles of Gd remain unknown. OBJECTIVE:The aim of this in vitro study was to investigate the effect of Gd on the development of fibrosis and calcification in cultured cells. METHODS:MC3T3-E1 cells (pre-osteoblastic cells), human adipose tissue-derived mesenchymal stem cells (HAMSCs), human subcutaneous preadipocytes, and human dermal fibroblasts (HDFs) were each cultured in differentiation medium with or without gadolinium chloride. Calcium deposition of MC3T3-E1 cells, HAMSCs, and HDFs was determined by alzarin red S staining. Adipogenic differentiation of human subcutaneous preadipocytes and HAMSCs was determined by oil red O staining. Fibrogenesis of HDFs was determined by real-time PCR to measure the mRNA expression of type I collagen. Cell proliferation was determined by MTS assay. RESULTS:Gd induced calcium deposition in MC3T3-E1 cells, HAMSCs and HDFs in osteogenic differentiation media. Gd did not induce adipogenic differentiation in human subcutaneous preadipocytes and HAMSCs. Gd did not increase the mRNA expression of type I collagen in HDFs, but did promote cell proliferation. CONCLUSIONS:We have demonstrated a direct effect of Gd on calcium deposition in cultured cells. The result will help us to understand the mechanism of abnormal calcification in NSF. |
| 巻・号 | 62(1) |
| ページ | 58-63 |
| 公開日 | 2011-4-1 |
| DOI | 10.1016/j.jdermsci.2011.01.009 |
| PII | S0923-1811(11)00031-4 |
| PMID | 21334857 |
| MeSH | 3T3 Cells Adipose Tissue / metabolism Animals Calcinosis / metabolism Calcium / metabolism Cell Differentiation Cell Proliferation Culture Media Dose-Response Relationship, Drug Fibrosis / pathology* Gadolinium / pharmacology* Humans Mesenchymal Stem Cells / cytology* Mice Nephrogenic Fibrosing Dermopathy / metabolism Reverse Transcriptase Polymerase Chain Reaction Skin / metabolism Skin / pathology* |
| IF | 3.681 |
| 引用数 | 7 |
| WOS 分野 | DERMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |