RRC ID 1858
Author Kageyama S, Liu H, Nagata M, Aoki F.
Title The role of ETS transcription factors in transcription and development of mouse preimplantation embryos.
Journal Biochem Biophys Res Commun
Abstract Embryonic transcription is a crucial process for the creation of new life. To clarify the mechanism of embryonic transcription, we investigated the expression and function of the erythroblast transformation specific (ETS) domain containing transcription factors (TFs) during preimplantation development in mice. The expression levels of several ETS TFs, i.e., etsrp71, elf3, and spic, increased after fertilization and remained at a high level until the blastocyst stage. To clarify the function of these TFs, we performed gene suppression using RNA interference, which revealed that they were involved in regulating development to the blastocyst stage. Furthermore, we found that suppression of ETS TFs affected the transcription of eIF-1A and oct3/4 genes whose expression is regulated by TATA-less promoters in the embryos. These results suggest that ETS TFs function in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
Volume 344(2)
Pages 675-9
Published 2006-6-2
DOI 10.1016/j.bbrc.2006.03.192
PII S0006-291X(06)00753-4
PMID 16630543
MeSH Animals Blastocyst / metabolism* Embryonic Development / physiology* Gene Expression Regulation, Developmental / physiology* Mice Proto-Oncogene Proteins c-ets / metabolism* Transcription, Genetic / physiology*
IF 2.705
Times Cited 13
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb