RRC ID 1861
Author Ishigaki H, Yamamoto Y, Ishida H, Kajino K, Itoh Y, Fujiyama Y, Ogasawara K.
Title Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
Journal Microbiol Immunol
Abstract Tumor-infiltrating stroma cells (TISC) as well as tumors themselves are thought to be involved in tumor-related immunosuppression, which is one of the critical mechanisms of tumor escape from immune surveillance. However, preparation of TISC is difficult because of the small proportion of TISC in established tumors. Thus, the cells thought to be involved in tumor-related immunosuppression are generally prepared from spleens or draining lymph nodes in tumor-bearing mice. In this study, we developed a method for directly preparing TISC from established tumors in order to analyze their function. Using green fluorescent protein (GFP) transgenic (Tg) mice and C57BL/6 mice transplanted with bone marrow (BM) cells of GFPTg mice, we detected three subpopulations of TISC: one is compatible with immature myeloid cells (ImC) derived from BM and the two other subpopulations, CD11b(+) cells and CD11b(-) cells, do not originate from BM. The TISC including these subpopulations but not each subpopulation independently after culturing with tumors in the presence of GM-CSF could suppress T cell proliferation induced by anti-CD3. In our system, tumors did not inhibit T cell responses directly, but unknown factors from tumors affected immunosuppression by TISC.
Volume 50(8)
Pages 655-62
Published 2006-1-1
DOI 10.1111/j.1348-0421.2006.tb03830.x
PII JST.JSTAGE/mandi/50.655
PMID 16924151
MeSH Animals Bone Marrow Cells / cytology Bone Marrow Cells / immunology* Bone Marrow Transplantation / immunology CD11b Antigen / immunology Enzyme-Linked Immunosorbent Assay Flow Cytometry Immune Tolerance Immunologic Surveillance Interleukin-10 / immunology Interleukin-10 / metabolism Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Transgenic Neoplasm Transplantation Stromal Cells / cytology Stromal Cells / immunology* T-Lymphocytes / immunology* Thy-1 Antigens / immunology Thymoma / immunology* Transforming Growth Factor beta / immunology Transforming Growth Factor beta / metabolism Transplantation Chimera* Tumor Escape
IF 1.442
Times Cited 1
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb