| RRC ID |
18613
|
| 著者 |
Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z.
|
| タイトル |
Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models.
|
| ジャーナル |
J Histochem Cytochem
|
| Abstract |
Xenograft transplantation of human tumor cells into immunodeficient mice is an important method to clarify the roles of specific molecules or chemicals in vivo. Recently, this method has been reported as a definitive examination to identify tumor stem cells. In this study, the authors compared the morphology and the quality and quantity of ribonucleic acid (RNA) and protein in paraffin-embedded tissues of nude mice implanted with human uterine cervical cancer cells, followed by fixation with commonly used fixatives, including 4% paraformaldehyde (PFA), 10% neutral buffered formalin (NBF), 20% NBF, and 99% ethanol (EtOH). The quality of the isolated RNA from PFA- and NBF-fixed paraffin-embedded tissues was high, while EtOH-fixed tissues showed degradation of RNA. NBF-fixed tissues showed excellent quality of morphology, but EtOH-fixed tissues showed contraction of cells. Immunohistochemical results showed differences depending on fixations. The 99% EtOH-fixed samples showed decreases of Ki-67 and VEGF-A immunoreactivities, but improved cytokeratin immunoreactivity. This study indicated that formalin fixation is better than alcohol fixation for RNA preservation in paraffin-embedded cancer cell implantation models. Immunohistochemical results differed markedly depending on fixation materials and antibodies; therefore, suitable fixations are needed to quantify and compare the results of immunohistochemical staining on cancer cell implanted nude mice tissues.
|
| 巻・号 |
59(1)
|
| ページ |
68-75
|
| 公開日 |
2011-1-1
|
| DOI |
10.1369/jhc.2010.957217
|
| PII |
jhc.2010.957217
|
| PMID |
20940453
|
| PMC |
PMC3201123
|
| MeSH |
Animals
Antigens / immunology
Antigens / metabolism
Cell Line, Tumor
Cell Transformation, Neoplastic*
Fixatives / metabolism
Humans
Male
Mice
Paraffin Embedding / methods*
Proteins / analysis
Proteins / isolation & purification
Proteins / metabolism*
RNA / analysis
RNA / isolation & purification
RNA / metabolism*
Tissue Fixation / methods*
|
| IF |
2.187
|
| 引用数 |
33
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Ca Ski(RCB1947) |
