RRC ID 18614
Author Sakaguchi T, Irie T, Kuwayama M, Ueno T, Yoshida A, Kawabata R.
Title Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5.
Journal Microbiol. Immunol.
Abstract Sendai virus (SeV), a pneumotropic virus of rodents, has an accessory protein, V, and the V protein has been shown to interact with MDA5, inhibiting IRF3 activation and interferon-β production. In the present study, interaction of the V protein with various IRF3-activating proteins including MDA5 was investigated in a co-immunoprecipitation assay. We also investigated interaction of mutant V proteins from SeVs of low pathogenicity with MDA5. The V protein interacted with at least retinoic acid inducible gene I, inhibitor of κB kinase epsilon and IRF3 other than MDA5. However, only MDA5 interacted with the V protein dependently on the C-terminal V unique (Vu) region, inhibiting IRF3 reporter activation. The Vu region has been shown to be important for viral pathogenicity. We thus focused on interaction of the V protein with MDA5. Point mutations in the Vu region destabilized the V protein or abolished the interaction with MDA5 when the V protein was stable. The V-R₃₂₀G protein was highly stable and interacted with MDA5, but did not inhibit activation of IRF3 induced by MDA5. Viral pathogenicity of SeV is related to the inhibitory effect of the V protein on MDA5, but is not always related to the binding of V protein with MDA5.
Volume 55(11)
Pages 760-7
Published 2011-11
DOI 10.1111/j.1348-0421.2011.00379.x
PMID 21851384
MeSH Animals Cell Line DEAD-box RNA Helicases / metabolism* Humans Immunoprecipitation Interferon Regulatory Factor-3 / metabolism Interferon-Induced Helicase, IFIH1 Mice Mutant Proteins / genetics Mutant Proteins / metabolism Protein Binding Protein Interaction Mapping* Trans-Activators / metabolism Viral Proteins / genetics Viral Proteins / metabolism*
IF 1.335
Times Cited 5
Human and Animal Cells 293T (RCB2202)