論文 - 詳細
| RRC ID | 18614 |
|---|---|
| 著者 | Sakaguchi T, Irie T, Kuwayama M, Ueno T, Yoshida A, Kawabata R. |
| タイトル | Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5. |
| ジャーナル | Microbiol Immunol |
| Abstract |
Sendai virus (SeV), a pneumotropic virus of rodents, has an accessory protein, V, and the V protein has been shown to interact with MDA5, inhibiting IRF3 activation and interferon-β production. In the present study, interaction of the V protein with various IRF3-activating proteins including MDA5 was investigated in a co-immunoprecipitation assay. We also investigated interaction of mutant V proteins from SeVs of low pathogenicity with MDA5. The V protein interacted with at least retinoic acid inducible gene I, inhibitor of κB kinase epsilon and IRF3 other than MDA5. However, only MDA5 interacted with the V protein dependently on the C-terminal V unique (Vu) region, inhibiting IRF3 reporter activation. The Vu region has been shown to be important for viral pathogenicity. We thus focused on interaction of the V protein with MDA5. Point mutations in the Vu region destabilized the V protein or abolished the interaction with MDA5 when the V protein was stable. The V-R₃₂₀G protein was highly stable and interacted with MDA5, but did not inhibit activation of IRF3 induced by MDA5. Viral pathogenicity of SeV is related to the inhibitory effect of the V protein on MDA5, but is not always related to the binding of V protein with MDA5. |
| 巻・号 | 55(11) |
| ページ | 760-7 |
| 公開日 | 2011-11-1 |
| DOI | 10.1111/j.1348-0421.2011.00379.x |
| PMID | 21851384 |
| MeSH | Animals Cell Line DEAD-box RNA Helicases / metabolism* Humans Immunoprecipitation Interferon Regulatory Factor-3 / metabolism Interferon-Induced Helicase, IFIH1 Mice Mutant Proteins / genetics Mutant Proteins / metabolism Protein Binding Protein Interaction Mapping* Trans-Activators / metabolism Viral Proteins / genetics Viral Proteins / metabolism* |
| IF | 1.566 |
| 引用数 | 11 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |