RRC ID 18636
著者 Yanagitai M, Itoh S, Kitagawa T, Takenouchi T, Kitani H, Satoh T.
タイトル Carnosic acid, a pro-electrophilic compound, inhibits LPS-induced activation of microglia.
ジャーナル Biochem Biophys Res Commun
Abstract In the previous studies, we reported that carnosic acid (CA) protects cortical neurons by activating the Keap1/Nrf2 pathway, which activation is initiated by S-alkylation of the critical cysteine thiol of the Keap1 protein by the "electrophilic"quinone-type CA. Here, we found that the pro-electrophilic CA inhibited the in vitro lipopolysaccharide (LPS)-induced activation of cells of the mouse microglial cell line MG6. LPS induced the expression of IL-1β and IL-6, typical inflammatory cytokines released from microglial cells. CA inhibited the NO production associated with a decrease in the level of inducible NO synthase. Neither CA nor LPS affected cell survival at the concentrations used here. These actions of CA seemed to be mediated by induction of phase 2 genes (gclc, gclm, nqo1 and xct). We propose that an inducer of phase 2 genes may be a critical regulator of microglial activation. Thus, CA is a unique pro-electrophilic compound that provides both a protective effect on neurons and an anti-inflammatory one on microglia through induction of phase 2 genes.
巻・号 418(1)
ページ 22-6
公開日 2012-2-3
DOI 10.1016/j.bbrc.2011.12.087
PII S0006-291X(11)02296-0
PMID 22214931
MeSH Abietanes / pharmacology* Amino Acid Transport System y+ / genetics Animals Cell Line G2 Phase / drug effects* G2 Phase / genetics Glutamate-Cysteine Ligase / genetics Interleukin-1beta / antagonists & inhibitors Interleukin-1beta / biosynthesis Interleukin-6 / antagonists & inhibitors Interleukin-6 / biosynthesis Lipopolysaccharides / immunology Mice Microglia / drug effects* Microglia / immunology* NAD(P)H Dehydrogenase (Quinone) / genetics Nitric Oxide / antagonists & inhibitors Nitric Oxide / biosynthesis Nitric Oxide Synthase Type II / antagonists & inhibitors Plant Extracts / pharmacology*
IF 2.985
引用数 25
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MG6(RCB2403)