Reference - Detail
| RRC ID | 18645 |
|---|---|
| Author | Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M. |
| Title | A novel hairless mouse model for malignant melanoma. |
| Journal | J Dermatol Sci |
| Abstract |
BACKGROUND:An appropriate animal model for malignant melanoma could be a strong tool to develop biomarkers through analysis of melanomagenesis. OBJECTIVE:Development of a novel animal model that spontaneously develops malignant melanoma with a high percentage. METHODS:We crossed oncogenic RET (RFP-RET)-carrying transgenic mice of line 304/B6 (RET-mice) with hairless mice (hr/hr) and newly established hairless RFP-RET-transgenic mice of line 304-hr/hr (HL-RET-mice). RESULTS:The HL-RET-mice developed hyperpigmented skin and benign melanocytic tumors without exception. More importantly, 63.8% (46/72) of the benign tumors were transformed to malignant melanoma in the HL-RET-mice. Mean time until the development of benign melanocytic tumors (2.4 months; n = 102) in the HL-RET-mice was about half of that in the original RET-mice (4.6 months; n = 20). Mean life span in the HL-RET-mice (9.7 months; n = 38) was also significantly (p < 0.01) shorter than that in the original RET-mice (10.8 months; n = 20). Since early development of tumors could contribute to shortening of the research period, HL-RET-mice could be a useful model for analysis of melanomagenesis. We then found that the expression level of Mps one binder kinase activator-like-2B (Mobkl2b) in benign tumors was higher than that in malignant melanoma in HL-RET-mice. Expression level of MOBKL2B in malignant melanoma cell lines was also lower than that in non-malignant melanocytic cells in mice and humans, suggesting that MOBKL2B could be a novel marker for malignant melanoma. CONCLUSION:We established a novel hairless RET-transgenic mouse line spontaneously developing cutaneous malignant melanomas from benign melanocytic tumors. This mouse model may be useful to find new candidates of melanoma-related molecule. |
| Volume | 65(3) |
| Pages | 207-12 |
| Published | 2012-3-1 |
| DOI | 10.1016/j.jdermsci.2011.10.010 |
| PII | S0923-1811(11)00315-X |
| PMID | 22169325 |
| MeSH | Animals Biomarkers, Tumor / metabolism Cell Transformation, Neoplastic / pathology Disease Models, Animal* Melanoma* / genetics Melanoma* / metabolism Melanoma* / pathology Mice Mice, Hairless* Mice, Transgenic Neoplasm Proteins / metabolism Proto-Oncogene Proteins c-ret / genetics Skin Neoplasms* / genetics Skin Neoplasms* / metabolism Skin Neoplasms* / pathology |
| IF | 3.681 |
| Times Cited | 8 |
| WOS Category | DERMATOLOGY |
| Resource | |
| Human and Animal Cells | G-361(RCB0991) COLO 679(RCB0989) HMV-II(RCB0777) SK-MEL-28(RCB1930) |