RRC ID 18651
Author Takahashi K, Sugiyama T, Tokoro S, Neri P, Mori H.
Title Inhibition of interferon-γ-induced nitric oxide production by 10-hydroxy-trans-2-decenoic acid through inhibition of interferon regulatory factor-8 induction.
Journal Cell Immunol
Abstract 10-Hydroxy-trans-2-decenoic acid (10H2DA) is a major lipid component of royal jelly, a honey bee secretion used to nourish the queen bee and young larvae. In this study, we examined the effect of 10H2DA on interferon (IFN)-γ-induced nitric oxide (NO) production. IFN-γ-induced NO production and activation of the inducible NO synthase promoter were significantly inhibited by 10H2DA. IFN-γ-induced phosphorylation of signal transducer and activator of transcription-1 was not affected by 10H2DA. In contrast, IFN-γ-induced tumor necrosis factor (TNF)-α production and nuclear factor (NF)-κB activation were inhibited by 10H2DA. IFN-γ-mediated induction of interferon regulatory factor (IRF)-8, but not IRF-1, was also inhibited by 10H2DA. IFN-γ-induced TNF-α production followed by activation of NF-κB is known to be essential for NO production. Together, 10H2DA inhibited IFN-γ-induced NO production by inhibiting IRF-8 induction and TNF-α production. 10H2DA might modulate IFN-γ-mediated cellular responses by inhibiting the induction of IRF-8 and IRF-8-dependent genes.
Volume 273(1)
Pages 73-8
Published 2012-1-1
DOI 10.1016/j.cellimm.2011.11.004
PII S0008-8749(11)00298-X
PMID 22177846
MeSH Animals Cell Line Enzyme Activation / immunology Fatty Acids / pharmacology Fatty Acids, Monounsaturated / pharmacology* Interferon Regulatory Factors / antagonists & inhibitors* Interferon Regulatory Factors / genetics Interferon Regulatory Factors / immunology Interferon-gamma / antagonists & inhibitors* Interferon-gamma / immunology Macrophages / drug effects* Macrophages / enzymology Macrophages / immunology Mice NF-kappa B / immunology Nitric Oxide / antagonists & inhibitors Nitric Oxide / biosynthesis* Nitric Oxide Synthase Type II / antagonists & inhibitors Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / immunology RNA, Messenger / chemistry RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction STAT1 Transcription Factor / immunology Transcription, Genetic / immunology Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / immunology
IF 4.078
Times Cited 10
Human and Animal Cells RAW 264(RCB0535)