| RRC ID |
18699
|
| 著者 |
Miyake M, Hayashi S, Iwasaki S, Chao G, Takahashi H, Watanabe K, Ohwada S, Aso H, Yamaguchi T.
|
| タイトル |
Possible role of TIEG1 as a feedback regulator of myostatin and TGF-beta in myoblasts.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Myostatin and TGF-beta negatively regulate skeletal muscle development and growth. Both factors signal through the Smad2/3 pathway. However, the regulatory mechanism of myostatin and TGF-beta signaling remains unclear. TGF-beta inducible early gene (TIEG) 1 is highly expressed in skeletal muscle and has been implicated in the modulation of TGF-beta signaling. These findings prompted us to investigate the effect of TIEG1 on myostatin and TGF-beta signaling using C2C12 myoblasts. Myostatin and TGF-beta induced the expression of TIEG1 and Smad7 mRNAs, but not TIEG2 mRNA, in proliferating C2C12 cells. When differentiating C2C12 myoblasts were stimulated by myostatin, TIEG1 mRNA was up-regulated at a late stage of differentiation. In contrast, TGF-beta enhanced TIEG1 expression at an early stage. Overexpression of TIEG1 prevented the transcriptional activation of Smad by myostatin and TGF-beta in both proliferating or differentiating C2C12 cells, but the expression of Smad2 and Smad7 mRNAs was not affected. Forced expression of TIEG1 inhibited myogenic differentiation but did not cause more inhibition than the empty vector in the presence of myostatin or TGF-beta. These results demonstrate that TIEG1 is one possible feedback regulator of myostatin and TGF-beta that prevents excess action in myoblasts.
|
| 巻・号 |
393(4)
|
| ページ |
762-6
|
| 公開日 |
2010-3-19
|
| DOI |
10.1016/j.bbrc.2010.02.077
|
| PII |
S0006-291X(10)00297-4
|
| PMID |
20171187
|
| MeSH |
Animals
Cell Line
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Feedback, Physiological*
Mice
Muscle Development*
Myoblasts / metabolism
Myoblasts / physiology*
Myostatin / genetics
Myostatin / metabolism*
Signal Transduction
Smad Proteins / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism*
Transforming Growth Factor beta / genetics
Transforming Growth Factor beta / metabolism*
|
| IF |
2.985
|
| 引用数 |
11
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
