RRC ID 18712
Author Yin X, Yan X, Yang Q, Cao H, Liang H.
Title Antitumor mechanism of recombinant murine interleukin-12 vaccine.
Journal Cancer Biother. Radiopharm.
Abstract This study was designed to establish an interleukin-12 (IL-12)-expressing murine Lewis lung carcinoma (LLC) cell vaccine (LLC/murine IL-12 [mIL-12]) and assess its antitumor efficacy and mechanism in vivo. The recombinant IL-12 plasmid was transfected into LLC cells and screened by G418, and positive clones were obtained. C57BL/6 tumor-bearing mouse model was established and tumor-bearing mice were randomly divided into three groups (n = 20), that is, treated with an intratumoral injection of phosphate-buffered solution, blank plasmid, or LLC/mIL-12 vaccine, respectively, at days 0, 7, and 14. Tumor size was measured before and after treatment. Tumor growth curve was plotted, cytolytic T lymphocyte (CTL) activity assay and natural killer (NK) cell activity assay were performed, CD4(+) and CD8(+) T lymphocyte were quantitated using flow cytometry, and the expression of interferon-gamma (IFN-gamma), IL-12, and interferon-inducible protein-10 (IP-10) in serum was detected by ELISA. Microvessel density was determined by immunohistochemistry after all mice were euthanized at day 21. The study revealed suppressed tumor growth, elevated levels of IFN-gamma, IP-10, and IL-12, augmented NK and CTL cell activities, and decreased microvessel density of tumor tissues. There were abundant CD4(+) and CD8(+) T lymphocyte infiltration in the vaccine group. This study demonstrated that the antitumor mechanism of LLC/mIL-12 vaccine was to promote IFN-gamma and IL-12 secretion, augment the NK and CTL cell activities, and decrease the microvessel density of tumors.
Volume 25(3)
Pages 263-8
Published 2010-6
DOI 10.1089/cbr.2010.0771
PMID 20578831
MeSH Animals CD4-Positive T-Lymphocytes / cytology CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / cytology CD8-Positive T-Lymphocytes / immunology Cancer Vaccines / administration & dosage Cancer Vaccines / immunology Cancer Vaccines / therapeutic use* Carcinoma, Lewis Lung / blood supply Carcinoma, Lewis Lung / immunology* Carcinoma, Lewis Lung / pathology Carcinoma, Lewis Lung / therapy* Cell Count Chemokine CXCL10 / blood Female Interferon-gamma / blood Interleukin-12 / blood Interleukin-12 / genetics Interleukin-12 / immunology* Interleukin-12 / therapeutic use* Killer Cells, Natural / immunology Mice Mice, Inbred C57BL Microvessels / pathology Recombinant Proteins / genetics Recombinant Proteins / immunology Recombinant Proteins / therapeutic use* Spleen / immunology T-Lymphocytes, Cytotoxic / immunology Vaccines, DNA / administration & dosage Vaccines, DNA / immunology Vaccines, DNA / therapeutic use*
IF 1.682
Times Cited 5
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING PHARMACOLOGY & PHARMACY ONCOLOGY
Resource
Human and Animal Cells LLC (RCB0558)