RRC ID 1874
著者 Aker J, Borst JW, Karlova R, de Vries S.
タイトル The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.
ジャーナル J Struct Biol
Abstract Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Förster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1.
巻・号 156(1)
ページ 62-71
公開日 2006-10-1
DOI 10.1016/j.jsb.2006.03.004
PII S1047-8477(06)00076-1
PMID 16621602
MeSH Arabidopsis / cytology Arabidopsis / enzymology* Arabidopsis Proteins / analysis Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Cell Membrane / metabolism* Endoplasmic Reticulum / metabolism Fluorescence Resonance Energy Transfer Luminescent Proteins / metabolism Phosphotransferases / metabolism* Protoplasts / cytology Protoplasts / metabolism
IF 3.071
引用数 34
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda09823 pda07243