Reference - Detail
RRC ID | 1878 |
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Author | Katoh A, Uenohara K, Akita M, Hashimoto T. |
Title | Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid. |
Journal | Plant Physiol |
Abstract |
NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize quinolinate from tryptophan, whereas other bacteria synthesize quinolinate from aspartate (Asp) using L-Asp oxidase and quinolinate synthase. We show here that Arabidopsis (Arabidopsis thaliana) uses the Asp-to-quinolinate pathway. The Arabidopsis L-Asp oxidase or quinolinate synthase gene complemented the Escherichia coli mutant defective in the corresponding gene, and T-DNA-based disruption of either of these genes, as well as of the gene coding for the enzyme quinolinate phosphoribosyltransferase, was embryo lethal. An analysis of functional green fluorescent protein-fused constructs and in vitro assays of uptake into isolated chloroplasts demonstrated that these three enzymes are located in the plastid. |
Volume | 141(3) |
Pages | 851-7 |
Published | 2006-7-1 |
DOI | 10.1104/pp.106.081091 |
PII | pp.106.081091 |
PMID | 16698895 |
PMC | PMC1489895 |
MeSH | Amino Acid Oxidoreductases / metabolism Amino Acid Oxidoreductases / physiology Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Aspartic Acid / metabolism* Escherichia coli / genetics Escherichia coli Proteins Multienzyme Complexes / metabolism Multienzyme Complexes / physiology NAD / biosynthesis* Pentosyltransferases / metabolism Pentosyltransferases / physiology Plastids / metabolism* Quinolinic Acid / metabolism |
IF | 6.902 |
Times Cited | 112 |
WOS Category | PLANT SCIENCES |
Resource | |
Arabidopsis / Cultured plant cells, genes | pda01728 pda01949 |