Reference - Detail
| RRC ID | 18889 |
|---|---|
| Author | Ichihara K, Tani A. |
| Title | Simple procedure for fatty acid analysis of glycerophospholipids in Escherichia coli and Saccharomyces cerevisiae. |
| Journal | J Biosci Bioeng |
| Abstract |
Rapid, convenient methods have been developed for fatty acid analysis of membrane glycerophospholipids in microorganisms. Fatty acid methyl esters derived from glycerophospholipids have been prepared directly from wet pellets of Escherichia coli cells or Saccharomyces cerevisiae spheroplasts without lipid extraction and fractionation in high yields under mild temperature conditions for analysis by gas chromatography. |
| Volume | 114(4) |
| Pages | 472-5 |
| Published | 2012-10-1 |
| DOI | 10.1016/j.jbiosc.2012.04.024 |
| PII | S1389-1723(12)00194-6 |
| PMID | 22652082 |
| MeSH | Chemical Fractionation Chromatography, Gas / methods* Escherichia coli / chemistry* Fatty Acids / analysis* Glycerophospholipids / chemistry* Saccharomyces cerevisiae / chemistry* Spheroplasts / chemistry Temperature |
| IF | 2.366 |
| Times Cited | 2 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Yeast | BY4947 |