RRC ID 18893
Author Kitagawa Y, Tohya Y, Ike F, Kajita A, Park SJ, Ishii Y, Kyuwa S, Yoshikawa Y.
Title Indirect ELISA and indirect immunofluorescent antibody assay for detecting the antibody against murine norovirus S7 in mice.
Journal Exp. Anim.
Abstract To evaluate murine norovirus (MNV) infection in laboratory mice, we attempted to develop an enzyme-linked immunosorbent assay (ELISA) system and an indirect immunofluorescent antibody (IFA) assay for detecting the anti-MNV-S7 antibody in mice. MNV-S7, which was isolated in Japan, was used in both assays. The antigen for ELISA was prepared by ultracentrifugation of culture supernatants of RAW 264 cells infected with MNV-S7. Positive sera were obtained from 6-week-old, female C57BL/6JJcl mice inoculated orally with MNV-S7. IFA against infected RAW 264 cells was able to discriminate positive sera from negative sera. Indirect ELISA was performed using 96-well ELISA plates coated with formalin-treated MNV-S7 antigen. In this ELISA system, mouse sera obtained 2 weeks after infection or later showed significantly high OD values and were judged positive. An equal level of anti-MNV-S7 antibody response was observed in BALB/cAJcl, C57BL/6JJcl, DBA/2JJcl, and Jcl:ICR mice; whereas, C3H/HeJJcl mice demonstrated slightly lower antibody production 4 weeks after infection. We also used this ELISA system to evaluate 77 murine serum samples obtained from 15 conventional mouse rooms in research facilities in Japan and found that approximately half of the serum samples contained antibody to MNV-S7. We found that some serum samples were negative for antibodies to mouse hepatitis virus and Mycoplasma pulmonis but positive for antibody to MNV-S7. The results suggest that the MNV infection is more prevalent than other infections such as mouse hepatitis virus and Mycoplasma pulmonis in conventional mouse colonies in Japan, as is the case in other areas of the world.
Volume 59(1)
Pages 47-55
Published 2010
DOI 10.1538/expanim.59.47
PII JST.JSTAGE/expanim/59.47
PMID 20224169
MeSH Animals Animals, Laboratory / immunology* Antibodies, Bacterial / blood Antibodies, Viral / blood* Enzyme-Linked Immunosorbent Assay / methods Enzyme-Linked Immunosorbent Assay / veterinary* Female Fluorescent Antibody Technique, Indirect / methods Fluorescent Antibody Technique, Indirect / veterinary* Mice / immunology* Mice, Inbred BALB C Mice, Inbred C3H / immunology Mice, Inbred C57BL / immunology Mice, Inbred DBA / immunology Murine hepatitis virus / immunology Mycoplasma pulmonis / immunology Norovirus / immunology*
IF 1.173
Times Cited 11
WOS Category VETERINARY SCIENCES ZOOLOGY
Resource
Human and Animal Cells RAW 264 (RCB0535)