Reference - Detail
| RRC ID | 18897 |
|---|---|
| Author | Bae J, Goto S, Mie M, Kobatake E. |
| Title | Construction of a thermostable cell adhesion protein for reverse transfection. |
| Journal | J Biotechnol |
| Abstract |
Transfection arrays are useful to analyze multiple genes at one time. In order to carry out gene transfection, cells are cultured on a plate on which genes are spotted to make extracellular matrix. However, this method is limited by low cell adhesion and transfection efficiency. To overcome these problems, we attempted to construct a novel extracellular matrix protein consisting of a variety of functional peptides. Here we fused the elastin derived peptide Ala-Pro-Gly-Val-Gly-Val (APGVGV) with the cell adhesive peptides, Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD). The resulting fusion proteins, E12PSGR, had high cell adhesive activity, transfection efficiency, and thermal stability. |
| Volume | 150(3) |
| Pages | 447-51 |
| Published | 2010-11-1 |
| DOI | 10.1016/j.jbiotec.2010.09.960 |
| PII | S0168-1656(10)01894-8 |
| PMID | 20934468 |
| MeSH | Animals Cell Adhesion* Cell Line Elastin / chemistry Elastin / genetics Elastin / metabolism Escherichia coli / genetics Extracellular Matrix Proteins / chemistry* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Fibronectins / chemistry Fibronectins / genetics Fibronectins / metabolism Hot Temperature Mice Oligopeptides / chemistry Oligopeptides / genetics Oligopeptides / metabolism Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / metabolism Protein Stability Recombinant Fusion Proteins / chemistry* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Transfection / methods* |
| IF | 3.503 |
| Times Cited | 4 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |