論文 - 詳細
| RRC ID | 18905 |
|---|---|
| 著者 | Yin XL, Yan X, Wen M, Peng ZP, Li SL. |
| タイトル | Synergistic antitumor effects of 131I-LC-1 IgM and IL-12 vaccine on Lewis lung carcinoma. |
| ジャーナル | Int Immunopharmacol |
| Abstract |
This study was designed to determine the antitumor effects of iodine-131 labeled monoclonal antibody LC-1 ((131)I-LC-1), interleukin-12 (IL-12) vaccine, or the combination of both on C57BL/6 mice bearing Lewis lung carcinoma (LLC) tumors. Tumor-bearing mice models were randomly divided into 4 groups that were respectively injected intratumorally with phosphate buffered solution (PBS), IL-12 vaccine gene therapy (GT), (131)I-LC-1 radioimmuno-therapy (RIT), or GT+RIT. Tumor volumes were measured before and after treatment. ELISA and RT-PCR determined the expression of IL-l2. LC-1 monoclonal antibody (Mab) was labeled with Na(131)I. Cytolytic T lymphocyte (CTL) activity assay, Natural Killer cell (NK) activity assay and apoptosis analysis were performed. Intratumoral (131)I-LC-1 injection leads to higher delivery of the antibody to the tumor. Tumor apoptosis occurred in the GT, RIT and GT+RIT groups. Tumor growth was inhibited in the GT, RIT and GT+RIT groups. Compared with other groups, the combination of GT+RIT up-regulated the expression of IL-l2 gene and inhibited the tumor growth more effectively than either GT or RIT alone (p<0.05). These results suggest that GT+RIT have the synergistic antitumor effects on tumor-bearing mice. |
| 巻・号 | 10(3) |
| ページ | 284-9 |
| 公開日 | 2010-3-1 |
| DOI | 10.1016/j.intimp.2009.11.012 |
| PII | S1567-5769(09)00362-2 |
| PMID | 19951748 |
| MeSH | Animals Apoptosis / drug effects Cancer Vaccines / therapeutic use* Carcinoma, Lewis Lung / drug therapy* Carcinoma, Lewis Lung / pathology Drug Synergism Enzyme-Linked Immunosorbent Assay Immunoglobulin M / metabolism Immunoglobulin M / therapeutic use* Interleukin-12 / immunology* Isotope Labeling Killer Cells, Natural / drug effects Killer Cells, Natural / immunology Mice Mice, Inbred C57BL Neoplasm Transplantation Plasmids / genetics RNA, Messenger / biosynthesis RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / drug effects Tissue Distribution Vaccines, DNA / immunology |
| IF | 3.943 |
| 引用数 | 0 |
| WOS 分野 | IMMUNOLOGY PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC(RCB0558) |