| RRC ID |
18905
|
| 著者 |
Yin XL, Yan X, Wen M, Peng ZP, Li SL.
|
| タイトル |
Synergistic antitumor effects of 131I-LC-1 IgM and IL-12 vaccine on Lewis lung carcinoma.
|
| ジャーナル |
Int Immunopharmacol
|
| Abstract |
This study was designed to determine the antitumor effects of iodine-131 labeled monoclonal antibody LC-1 ((131)I-LC-1), interleukin-12 (IL-12) vaccine, or the combination of both on C57BL/6 mice bearing Lewis lung carcinoma (LLC) tumors. Tumor-bearing mice models were randomly divided into 4 groups that were respectively injected intratumorally with phosphate buffered solution (PBS), IL-12 vaccine gene therapy (GT), (131)I-LC-1 radioimmuno-therapy (RIT), or GT+RIT. Tumor volumes were measured before and after treatment. ELISA and RT-PCR determined the expression of IL-l2. LC-1 monoclonal antibody (Mab) was labeled with Na(131)I. Cytolytic T lymphocyte (CTL) activity assay, Natural Killer cell (NK) activity assay and apoptosis analysis were performed. Intratumoral (131)I-LC-1 injection leads to higher delivery of the antibody to the tumor. Tumor apoptosis occurred in the GT, RIT and GT+RIT groups. Tumor growth was inhibited in the GT, RIT and GT+RIT groups. Compared with other groups, the combination of GT+RIT up-regulated the expression of IL-l2 gene and inhibited the tumor growth more effectively than either GT or RIT alone (p<0.05). These results suggest that GT+RIT have the synergistic antitumor effects on tumor-bearing mice.
|
| 巻・号 |
10(3)
|
| ページ |
284-9
|
| 公開日 |
2010-3-1
|
| DOI |
10.1016/j.intimp.2009.11.012
|
| PII |
S1567-5769(09)00362-2
|
| PMID |
19951748
|
| MeSH |
Animals
Apoptosis / drug effects
Cancer Vaccines / therapeutic use*
Carcinoma, Lewis Lung / drug therapy*
Carcinoma, Lewis Lung / pathology
Drug Synergism
Enzyme-Linked Immunosorbent Assay
Immunoglobulin M / metabolism
Immunoglobulin M / therapeutic use*
Interleukin-12 / immunology*
Isotope Labeling
Killer Cells, Natural / drug effects
Killer Cells, Natural / immunology
Mice
Mice, Inbred C57BL
Neoplasm Transplantation
Plasmids / genetics
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / drug effects
Tissue Distribution
Vaccines, DNA / immunology
|
| IF |
3.943
|
| 引用数 |
0
|
|
WOS 分野
|
IMMUNOLOGY
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
LLC(RCB0558) |
