| RRC ID |
18906
|
| 著者 |
Matsuo J, Kobayashi M, Nakamura S, Mizutani Y, Yao T, Hirai I, Yamamoto Y, Yamaguchi H.
|
| タイトル |
Stability of Chlamydophila pneumoniae in a harsh environment without a requirement for acanthamoebae.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
The effect of actual interactions between Chlamydophila pneumoniae and amoebae (Acanthamoeba) on the survival of C. pneumoniae was investigated. C. pneumoniae and amoebae were detected in 75 soil samples by IFU assay and PCR. Although C. pneumoniae could not be cultured, the DNA prevalence of C. pneumoniae and amoebae in natural soil was 20% and 92% (no correlation between the prevalence of DNA was observed). The viability of C. pneumoniae spiked in autoclaved soil was assessed by IFU assay and RT-PCR. Although the number of infective progeny decreased for three days, transcripts of C. pneumoniae were detected for up to 98 days independently of amoebae. The stability of C. pneumoniae in liquid medium was also assessed by IFU assay and transmission electron microscopy. The bacteria could survive at 15 degrees C for 14 days independently of amoebae. Bacteria cultured without amoebae were confirmed to have normal structures. Thus, the presence of amoebae has no effect on C. pneumoniae survival, and the bacteria can survive in the absence of host cells for an extended period of time.
|
| 巻・号 |
54(2)
|
| ページ |
63-73
|
| 公開日 |
2010-2-1
|
| DOI |
10.1111/j.1348-0421.2009.00182.x
|
| PII |
MIM182
|
| PMID |
20377739
|
| MeSH |
Acanthamoeba / growth & development
Acanthamoeba / microbiology*
Base Sequence
Chlamydia Infections / microbiology
Chlamydophila pneumoniae / genetics
Chlamydophila pneumoniae / growth & development*
Chlamydophila pneumoniae / isolation & purification
Disease Reservoirs / microbiology
Host-Pathogen Interactions
Humans
Microbial Viability*
Molecular Sequence Data
Soil / parasitology
Soil Microbiology
|
| IF |
1.566
|
| 引用数 |
7
|
|
WOS 分野
|
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HEp-2(RCB1889) |
