論文 - 詳細
| RRC ID | 1892 |
|---|---|
| 著者 | Hayashi K, Kawaide H, Notomi M, Sakigi Y, Matsuo A, Nozaki H. |
| タイトル | Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens. |
| ジャーナル | FEBS Lett |
| Abstract |
ent-Kaurene is the key intermediate in biosynthesis of gibberellins (GAs), plant hormones. In higher plants, ent-kaurene is synthesized successively by copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) from geranylgeranyl diphosphate (GGDP). On the other hand, fungal ent-kaurene synthases are bifunctional cyclases with both CPS and KS activity in a single polypeptide. The moss Physcomitrella patens is a model organism for the study of genetics and development in an early land plant. We identified ent-kaurene synthase (PpCPS/KS) from P. patens and analyzed its function. PpCPS/KS cDNA encodes a 101-kDa polypeptide, and shows high similarity with CPSs and abietadiene synthase from higher plants. PpCPS/KS is a bifunctional cyclase and, like fungal CPS/KS, directly synthesizes the ent-kaurene skeleton from GGDP. PpCPS/KS has two aspartate-rich DVDD and DDYFD motifs observed in CPS and KS, respectively. The mutational analysis of two conserved motifs in PpCPS/KS indicated that the DVDD motif is responsible for CPS activity (GGDP to CDP) and the DDYFD motif for KS activity (CDP to ent-kaurene and ent-16alpha-hydroxykaurene). |
| 巻・号 | 580(26) |
| ページ | 6175-81 |
| 公開日 | 2006-11-13 |
| DOI | 10.1016/j.febslet.2006.10.018 |
| PII | S0014-5793(06)01224-5 |
| PMID | 17064690 |
| MeSH | Alkyl and Aryl Transferases / genetics* Alkyl and Aryl Transferases / physiology* Amino Acid Motifs Amino Acid Sequence Bryophyta / enzymology* Mutagenesis, Site-Directed Phylogeny Plant Proteins Sequence Alignment |
| IF | 3.057 |
| 引用数 | 119 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdp33101 |