| RRC ID |
18921
|
| 著者 |
Umebayashi M, Makizono T, Ichihara H, Matsumoto Y, Ueoka R.
|
| タイトル |
Inhibitory effects of cationic hybrid liposomes on the growth of human renal cell carcinoma.
|
| ジャーナル |
Anticancer Res
|
| Abstract |
Cationic hybrid liposomes (HL) can be prepared by simply ultrasonicating a mixture of L-alpha-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene (21)dodecyl ether (C(12)(EO)(21)) and O,O'-ditetradecanoyl-N-(alpha-trimethylammonioacetyl) diethanolamine chloride (2C(14)ECl) in a buffer solution. The inhibitory effects of cationic HL containing 2C(14)ECl on the growth of OS-RC-2 human renal carcinoma cells in vitro and in vivo were examined. The 50% inhibitory concentration of cationic HL was remarkably reduced compared with that of DMPC liposomes. Induction of apoptosis by cationic HL in OS-RC-2 cells was verified on the basis of flow cytometric analysis and fluorescence microscopy in vitro. In addition, the effects on survival of cationic HL along with apoptosis in vivo were obtained using a mouse model of renal carcinoma. Chemotherapy with drug-free cationic HL for renal cell carcinoma was developed for the first time through the interaction between cationic HL and anionic surface region of tumor cell membranes, without any side-effects. The results obtained might be applied in chemotherapies used at present for patients with renal carcinoma.
|
| 巻・号 |
30(2)
|
| ページ |
327-37
|
| 公開日 |
2010-2-1
|
| PII |
30/2/327
|
| PMID |
20332436
|
| MeSH |
Animals
Apoptosis / drug effects
Blotting, Western
Carcinoma, Renal Cell / drug therapy*
Carcinoma, Renal Cell / metabolism
Carcinoma, Renal Cell / pathology*
Caspases / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects*
Dimyristoylphosphatidylcholine / pharmacology*
Female
Flow Cytometry
Hemolysis / drug effects
Humans
In Situ Nick-End Labeling
Kidney Neoplasms / drug therapy*
Kidney Neoplasms / metabolism
Kidney Neoplasms / pathology*
Liposomes*
Membrane Potential, Mitochondrial / drug effects
Mice
Mice, Inbred BALB C
Mice, Nude
|
| IF |
1.994
|
| 引用数 |
9
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
OS-RC-2(RCB0735) |
