論文 - 詳細
| RRC ID | 18945 |
|---|---|
| 著者 | Hirai T, Kaneshige K, Kurosaki T, Nishio H. |
| タイトル | Functional expression of 5-HT2A receptor in osteoblastic MC3T3-E1 cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
In the previous study, we reported the gene expression for proteins related to the function of 5-hydroxytryptamine (5-HT, serotonin) and elucidated the expression patterns of 5-HT(2) receptor subtypes in mouse osteoblasts. In the present study, we evaluated the possible involvement of 5-HT receptor subtypes and its inactivation system in MC3T3-E1 cells, an osteoblast cell line. DOI, a 5-HT(2A) and 5-HT(2C) receptor selective agonist, as well as 5-HT concentration-dependently increased proliferative activities of MC3T3-E1 cells in their premature period. This effect of 5-HT on cell proliferation were inhibited by ketanserin, a 5-HT(2A) receptor specific antagonist. Moreover, both DOI-induced cell proliferation and phosphorylation of ERK1 and 2 proteins were inhibited by PD98059 and U0126, selective inhibitors of MEK in a concentration-dependent manner. Furthermore, treatment with fluoxetine, a 5-HT specific re-uptake inhibitor which inactivate the function of extracellular 5-HT, significantly increased the proliferative activities of MC3T3-E1 cells in a concentration-dependent manner. Our data indicate that 5-HT fill the role for proliferation of osteoblast cells in their premature period. Notably, 5-HT(2A) receptor may be functionally expressed to regulate mechanisms underlying osteoblast cell proliferation, at least in part, through activation of ERK/MAPK pathways in MC3T3-E1 cells. |
| 巻・号 | 396(2) |
| ページ | 278-82 |
| 公開日 | 2010-5-28 |
| DOI | 10.1016/j.bbrc.2010.04.078 |
| PII | S0006-291X(10)00747-3 |
| PMID | 20399747 |
| MeSH | Amphetamines / pharmacology Animals Cell Line Cell Proliferation* Mice Mitogen-Activated Protein Kinase 1 / metabolism* Mitogen-Activated Protein Kinase 3 / metabolism* Osteoblasts / drug effects Osteoblasts / metabolism* Phosphorylation Receptor, Serotonin, 5-HT2A / biosynthesis* Serotonin 5-HT2 Receptor Antagonists Serotonin Receptor Agonists / pharmacology |
| IF | 2.985 |
| 引用数 | 9 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |