RRC ID 18982
Author Choi BH, Choi YS, Kang DG, Kim BJ, Song YH, Cha HJ.
Title Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides.
Journal Biomaterials
Abstract Adhesion of cells to surfaces is a basic and important requirement in cell culture and tissue engineering. Here, we designed artificial extracellular matrix (ECM) mimics for efficient cellular attachment, based on mussel adhesive protein (MAP) fusion with biofunctional peptides originating from ECM materials, including fibronectin, laminin, and collagen. Cellular behaviors, including attachment, proliferation, spreading, viability, and differentiation, were investigated with the artificial ECM material-coated surfaces, using three mammalian cell lines (pre-osteoblast, chondrocyte, and pre-adipocyte). All cell lines examined displayed superior attachment, proliferation, spreading, and survival properties on the MAP-based ECM mimics, compared to other commercially available cell adhesion materials, such as poly-L-lysine and the naturally extracted MAP mixture. Additionally, the degree of differentiation of pre-osteoblast cells on MAP-based ECM mimics was increased. These results collectively demonstrate that the artificial ECM mimics developed in the present work are effective cell adhesion materials. Moreover, we expect that the MAP peptide fusion approach can be extended to other functional tissue-specific motifs.
Volume 31(34)
Pages 8980-8
Published 2010-12-1
DOI 10.1016/j.biomaterials.2010.08.027
PII S0142-9612(10)01049-5
PMID 20832110
MeSH Animals Apoptosis / drug effects Biomimetic Materials / pharmacology* Cell Adhesion / drug effects Cell Differentiation / drug effects Cell Line Cell Movement / drug effects Cell Proliferation / drug effects Extracellular Matrix / chemistry* Mice Osteoblasts / cytology Osteoblasts / drug effects Peptides / isolation & purification Peptides / metabolism* Proteins / metabolism* Recombinant Fusion Proteins / metabolism* Surface Properties / drug effects
IF 10.273
Times Cited 65
Human and Animal Cells MC3T3-E1 (RCB1126) ATDC5 (RCB0565)