RRC ID 18993
Author Moore NM, Lin NJ, Gallant ND, Becker ML.
Title The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation.
Journal Biomaterials
Abstract In this study, we report the use of surface immobilized peptide concentration gradient technology to characterize MC3T3-E1 osteoblast cell response to osteogenic growth peptide (OGP), a small peptide found naturally in human serum at mumol/L concentrations. OGP was coupled to oxidized self assembled monolayer (SAM) gradients by a polyethylene oxide (PEO) linker using click chemistry. After 4h incubation with MC3T3-E1 cells, OGP functionalized surfaces had higher cell attachment at low peptide concentrations compared to control gradients. By day 3, OGP gradient substrates had higher cell densities compared to control gradients at all concentrations. MC3T3-E1 cell doubling time was 35% faster on OGP substrates relative to SAM gradients alone, signifying an appreciable increase in cell proliferation. This increase in cell proliferation, or decrease in doubling time, due to OGP peptide was reduced by day 7. Hence, immobilized OGP increased cell proliferation from 0 days to 3 days at all densities indicating it may be useful as a proliferative peptide that can be used in tissue engineering substrates.
Volume 31(7)
Pages 1604-11
Published 2010-3-1
DOI 10.1016/j.biomaterials.2009.11.011
PII S0142-9612(09)01223-X
PMID 19932505
MeSH Animals Biomarkers / metabolism Cell Adhesion / drug effects Cell Count Cell Proliferation / drug effects Cell Shape / drug effects Chemistry, Organic / methods* Collagen Type I / genetics Collagen Type I / metabolism Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Gene Expression Regulation / drug effects Histones / chemical synthesis* Histones / chemistry Histones / pharmacology* Humans Immobilized Proteins / pharmacology* Intercellular Signaling Peptides and Proteins / chemical synthesis* Intercellular Signaling Peptides and Proteins / chemistry Intercellular Signaling Peptides and Proteins / pharmacology* Mice Osteoblasts / cytology* Osteoblasts / drug effects* Osteoblasts / metabolism Substrate Specificity / drug effects Surface Properties / drug effects
IF 10.317
Times Cited 70
WOS Category ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
Resource
Human and Animal Cells MC3T3-E1(RCB1126)