RRC ID 19004
Author Lee CM, Sieo CC, Cheah YK, Abdullah N, Ho YW.
Title Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting.
Journal J. Sci. Food Agric.
Abstract BACKGROUND:Four repetitive element sequence-based polymerase chain reaction (rep-PCR) methods, namely repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), polytrinucleotide (GTG)₅ -PCR and BOX-PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep-PCR methods and their combination (composite rep-PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D).
RESULTS:Species-specific and strain-specific profiles were observed from rep-PCR. From the numerical analysis of composite rep-PCR, BOX-PCR, (GTG)₅ -PCR, REP-PCR and ERIC-PCR, D values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep-PCR analysis was the most discriminative method, with eight Lactobacillus strains, namely L. brevis ATCC 14869(T) , L. reuteri C 10, L. reuteri ATCC 23272(T) , L. gallinarum ATCC 33199(T) , L. salivarius ATCC 11741(T) , L. salivarius I 24, L. panis JCM 11053(T) and L. panis C 17, being differentiated at the strain level.
CONCLUSION:Composite rep-PCR analysis is potentially a useful fingerprinting method to discriminate probiotic Lactobacillus strains isolated from the gastrointestinal tract of chickens.
Volume 92(3)
Pages 660-6
Published 2012-2
DOI 10.1002/jsfa.4627
PMID 21919004
MeSH Animals Chickens / microbiology DNA Fingerprinting / veterinary DNA, Bacterial / metabolism* Diet / veterinary* Gastrointestinal Tract / microbiology Intestinal Mucosa / microbiology Inverted Repeat Sequences Lactobacillus / classification* Lactobacillus / genetics Lactobacillus / isolation & purification Lactobacillus / metabolism Malaysia Molecular Typing / methods Molecular Typing / veterinary Phylogeny Polymerase Chain Reaction / veterinary Probiotics / classification* Probiotics / isolation & purification Probiotics / metabolism Repetitive Sequences, Nucleic Acid Species Specificity
IF 2.379
Times Cited 1
General Microbes JCM 11053